Multidirectional Effects of Red Clover (Trifolium pratense L.) in Support of Menopause Therapy.

Red clover is a raw material of interest primarily due to its isoflavone content. However, other groups of compounds may affect the pleiotropic biological effects of this raw material. It is used to alleviate menopausal symptoms, but the fact that there are many varieties of this plant that can be grown makes it necessary to compare the biological activity and phytochemical composition of this plant. Also of interest are the differences between the leaves and flowers of the plant. The aim of this study was to evaluate the properties of the leaves and flowers of six clover varieties-'Tenia', 'Atlantis', 'Milena', 'Magellan', 'Lemmon' and 'Lucrum'-with respect to their ability to inhibit α-glucosidase, lipase, collagenase and antioxidant activity. Therefore, the contents of polyphenols and the four main isoflavones-genistein, daidzein, biochanin and formononetin-were assessed. The study was complemented by testing for permeability through a model membrane system (PAMPA). Principal component analysis (PCA) identified a relationship between activity and the content of active compounds. It was concluded that antioxidant activity, inhibition of glucosidase, collagenase and lipase are not correlated with isoflavone content. A higher content of total polyphenols (TPC) was determined in the flowers of red clover while a higher content of isoflavones was determined in the leaves of almost every variety. The exception is the 'Lemmon' variety, characterized by high isoflavone content and high activity in the tests conducted.

Mutual Effect of Gypsum and Potassium on Nutrient Productivity in the Alfalfa-Grass Sward-A Case Study.

It was assumed that the production of alfalfa in soils naturally poor in available nutrients, such as potassium (K) and calcium (Ca), depends on the use of fertilizers. This hypothesis was validated in an experiment with an alfalfa-grass mixture carried out in 2012, 2013 and 2014 on soil formed from loamy sand that had a low content of available Ca and K. The two-factor experiment consisted of two levels of applied gypsum as a source of Ca (0, 500 kg ha-1) and five levels of PK fertilizers (absolute control, P60K0, P60K30, P60K60 and P60K120). The total yield of the sward was determined by the main seasons of alfalfa-grass sward use. Gypsum application increased the yield by 1.0 t ha-1. The highest yield of 14.9 t ha-1 was obtained on the plot fertilized with P60K120. Based on the nutrient content in the sward, it was shown that the main yield predictor was the content of K in the first cut of sward use. The reliable yield predictors, based on the total accumulation of nutrients in the sward, turned out to be K, Mg and Fe. The nutritional quality of the alfalfa-grass fodder, based on the K/Ca + Mg ratio, depended mainly on the season of the sward use, which was substantially deteriorated by the K fertilizer. Gypsum did not control this process. The productivity of the nutrients taken up by the sward depended on the accumulated K. Its yield-forming effect was significantly limited by manganese deficiency. The use of gypsum positively affected the uptake of micronutrients, consequently increasing their unit productivity, especially of manganese. Optimization of the production of alfalfa-grass mixtures in soils poor in basic nutrients requires micronutrients to be taken into account. Their uptake by plants can be limited by high doses of basic fertilizers.

SPAD Leaf Greenness Index: Green Mass Yield Indicator of Maize (Zea mays L.), Genetic and Agriculture Practice Relationship.

The study presents the results of two field studies (Experiment I, Experiment II), whose aim was to assess the impact of agriculture factors on maize green mass and leaf greenness index (Soil and Plant Analysis Development, SPAD) in critical growth stages, as well as to determine the relationship between the SPAD index and the yield of green maize for ensiling. It was shown that thermal and humidity conditions in maize growing seasons determined the value of the SPAD leaf greenness index and the yield of maize harvested for silage. Row application of mineral fertilizer (N, NP) and selection of "stay-green" varieties guarantee a higher yield of maize green mass. Growing maize in direct sowing reduces chlorophyll content expressed in SPAD units, thereby reducing plant nitrogen condition, which significantly decreases the yield of biomass intended for silage. The SPAD leaf greenness index determined in critical stages of maize growth can be considered as a yield predictor of green mass for ensiling. The examined maize cultivars were divided into two groups on the basis of hierarchically grouping using the unweighted pair group method of arithmetic means. The first group comprised cultivars SY Cooky and Drim "stay-green," while the second one included cultivars ES Paroli "stay-green" and ES Palazzo.

Dry Matter Yield of Maize (Zea mays L.) as an Indicator of Mineral Fertilizer Efficiency.

This study presents the results of 3-year field trials, whose purpose was to assess the dynamics of dry matter accumulation by maize depending on the placement depth of a two-component (NP) mineral fertilizer in the soil layer, type of nitrogen fertilizer and date of its application. Weather conditions, mainly thermal in the early growing season, had a significant effect on maize responses to placement depth of phosphorus starting dose in the soil profile. In the initial stage of maize development, the temperature determined plant growth to a significantly higher extent than the sum of rainfall. The dry matter yield of ears and whole plants showed a clear reaction to starter phosphorus fertilization, but the effect of the depth of fertilizer placement varied over the years, indicating a depth of 5 cm and 10 cm as advisable and recommended for agricultural practice. The PFPFN (partial factor productivity of fertilizer nitrogen) and PFPFP (partial factor productivity of fertilizer phosphorus) indices confirmed the significant effect of fertilizer (NP) placement in the soil profile, indicating row fertilizer application (regardless of the depth) as recommended to improve the efficiency of maize fertilization. The SPAD (soil plant analysis development) leaf greenness index turned out to be a sensitive indicator of maize response to fertilizer (NP) placement depth in the soil profile.

Comparison of Soil Bioconditioners and Standard Fertilization in Terms of the Impact on Yield and Vitality of Lolium Perenne and Soil Biological Properties.

The aim of the study was to compare the influence of selected soil bioconditioners and traditional fertilisation P+K+Ca on the vitality and yield of Lolium perenne and on the microbiological state of soil. The study was conducted between 2008 and 2009 - it was based on a field experiment started in 2006, in Brody, at the Agricultural Experiment Station of the Poznan University of Life Sciences, Poland. The factorial experiment was conducted in a randomized block design, with three replications. Two experimental factors were used: - non-nitrogen fertilisation (Physio-Mescal G18, PRP-SOL, Effective Microorganisms, Effective Microorganisms + Ca, P+K+Ca); - nitrogen fertilisation (N0 and N200 kg ha-1). The following parameters were measured: the yield of dry matter of perennial ryegrass, the plants' vitality (chlorophyll concentration), the count of selected groups of soil microorganisms (heterotrophic, oligotrophic, copiotrophic bacteria, actinobacteria, fungi), soil enzymatic activity (dehydrogenases, acid phosphatase), and soil pH. The experiment showed that the bioconditioners were not an alternative to traditional mineral fertilisation, especially to nitrogen fertilisation, as a basic yield factor, but they could be a very valuable supplement to this fertilisation, and help to maintain the right biological potential of soil and its fertility, especially in the places where no manure or other non-chemical fertilisers are used.