RESUMO
The objective of this work was to comparatively study the tissue tropism and the associated pathology of 2 autochthonous small ruminant lentivirus (SRLV) field strains using an experimental infection in sheep through the bone marrow. Fifteen male, SRLV-free lambs of the Rasa Aragonesa breed were inoculated with strain 697 (nervous tissue origin, animals A1-A6), with strain 496 (articular origin, animals B1-B6), or with uninfected culture medium (C1-C3). Clinical, serologic, and polymerase chain reaction (PCR) evaluations were performed periodically. Two lambs from each infected group and a control animal were euthanized at 134, 273, and 319 days postinfection. Tissues were analyzed by gross and histopathologic evaluation; immunohistochemistry for CD3, CD4, CD8, CD68, and FoxP3 cell markers; lung morphometric evaluation; and tissue proviral quantification by PCR. All infected animals became positive either by enzyme-linked immunosorbent assay and/or PCR, with group B lambs showing the highest serologic values and more consistently positive PCR reactions. Group A lambs showed representative lung lesions but only mild histopathologic changes in the central nervous system (CNS) or in carpal joints. Contrarily, group B lambs demonstrated intense carpal arthritis and interstitial pneumonia but an absence of lesions in the CNS. Proviral copies in tissues were detected only in group B lambs. Experimental infection with these SRLV strains indicates that strain 496 is more virulent than strain 697 and more prone to induce arthritis, whereas strain 697 is more likely to reproduce encephalitis in Rasa Aragonesa lambs. Host factors as well as viral factors are responsible for the final clinicopathologic picture during SRLV infections.
Assuntos
Medula Óssea/virologia , Infecções por Lentivirus/veterinária , Lentivirus Ovinos-Caprinos/patogenicidade , Tropismo Viral , Animais , Medula Óssea/patologia , Sistema Nervoso Central/patologia , Sistema Nervoso Central/virologia , Ensaio de Imunoadsorção Enzimática/veterinária , Articulações/patologia , Articulações/virologia , Infecções por Lentivirus/patologia , Infecções por Lentivirus/virologia , Pulmão/patologia , Pulmão/virologia , Masculino , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Ovinos/virologia , Tropismo Viral/fisiologiaRESUMO
We describe the clinicopathologic features of an arthritis outbreak in sheep induced by small ruminant lentivirus (SRLV), linked to the presence of a new SRLV isolate phylogenetically assigned to caprine arthritis encephalitis virus-like subgroup B2. Thirteen SRLV seropositive Rasa Aragonesa adult ewes were selected from 5 SRLV highly infected flocks (mean seroprevalence, 90.7%) for presenting uni- or bilateral chronic arthritis in the carpal joint. A complete study was performed, including symptomatology, histopathology, immunocytochemistry, immunohistochemistry, in situ hybridization, and microbiology. The carpus was the joint almost exclusively affected, with 10 sheep (76%) showing a moderate increase in carpal joint size (diameter range, 18-20 cm; normal range, 15-16 cm) without signs of locomotion problems and with 3 ewes (23%) showing severe inflammation with marked increase in diameter (21-24 cm), pain at palpation, and abnormal standing position. Grossly, chronic proliferative arthritis was observed in affected joints characterized by an increased thickness of the synovial capsule and synovial membrane proliferation. Microscopically, synovial membrane inflammation and proliferation and hyperplasia of synoviocytes were observed. More positive cases of SLRV infection were detected by immunocytochemistry of articular fluid than of bronchoalveolar lavage fluid. Immunohistochemistry and in situ hybridization also detected positive cells in the subsynovial connective tissue, lung, mediastinal lymph node, mammary gland, and mammary lymph node. All animals were negative for the presence of Mycoplasma or other bacteria in the articular space. The present outbreak likely represents an adaptation of a caprine virus to sheep. Our results underline the importance of the arthritis induced by SRLV in sheep, a clinical form that might be underestimated.
Assuntos
Artrite/veterinária , Infecções por Lentivirus/veterinária , Lentivirus/fisiologia , Doenças dos Ovinos/patologia , Animais , Artrite/patologia , Artrite/virologia , Vírus da Artrite-Encefalite Caprina/genética , Vírus da Artrite-Encefalite Caprina/fisiologia , Genótipo , Lentivirus/genética , Infecções por Lentivirus/patologia , Infecções por Lentivirus/virologia , Filogenia , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/virologia , Especificidade da Espécie , Membrana Sinovial/virologiaRESUMO
The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhibition in cells transduced with ovine TRIM5α restored restricted viral DNA synthesis, suggesting a conserved mechanism of restriction. Identification of TRIM5α active molecular species may open new prophylactic strategies against lentiviral infections.
Assuntos
Proteínas de Transporte/metabolismo , Doenças das Cabras/imunologia , Doenças dos Ovinos/imunologia , Vírus Visna-Maedi/fisiologia , Visna/metabolismo , Sequência de Aminoácidos , Animais , Proteínas de Transporte/química , Proteínas de Transporte/genética , Doenças das Cabras/genética , Doenças das Cabras/metabolismo , Doenças das Cabras/virologia , Cabras , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Ovinos , Doenças dos Ovinos/genética , Doenças dos Ovinos/metabolismo , Doenças dos Ovinos/virologia , Visna/genética , Visna/virologiaAssuntos
Aneurisma Roto/cirurgia , Aneurisma/cirurgia , Anormalidades Cardiovasculares/cirurgia , Transtornos de Deglutição/cirurgia , Procedimentos Endovasculares , Artéria Subclávia/anormalidades , Aneurisma/complicações , Aneurisma Roto/complicações , Anormalidades Cardiovasculares/complicações , Transtornos de Deglutição/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Artéria Subclávia/cirurgiaRESUMO
Small ruminant lentiviruses (SRLV) are widely spread in many countries, including Spain. However, little is known about the genetic characteristics of Spanish goat and sheep SRLV. In this study, segments from three genomic regions (pol, gag-p25 and LTR) were amplified using DNA isolated from three Spanish autochthonous sheep (one) and goats (two). Animals (one per flock) belonged to distantly located, single-species flocks (goat or sheep). Sequence analysis showed conservation of regions that are putatively relevant to viral survival. Sequences of Spanish goat and sheep SRLV were allocated into phylogenetic trees (phylograms) with known SRLV groups. The phylograms corresponding to the pol, gag-p25 and LTR regions analyzed presented a compatible topology. This showed that Spanish caprine and ovine SRLV sequences belonged to the A or D phylogenetic groups and were closer to sheep SRLV prototypes (A1 group) than to goat SRLV prototypes (B or C groups), according to the current classification [Shah, C., Boni, J., Huder, J.B., Vogt, H.R., Muhlherr, J., Zanoni, R., Miserez, R., Lutz, H., Schupbach, J., 2004a. Phylogenetic analysis and reclassification of caprine and ovine lentiviruses based on 104 new isolates: evidence for regular sheep-to-goat transmission and worldwide propagation through livestock trade. Virology 319 (1), 12-26]. It was not possible to amplify in the three genetic regions the expected fragment in additional Spanish caprine and ovine SRLV proviral DNA sequences with the PCR primers used. This suggests that there is heterogeneity at the primer binding site among Spanish SRLV sequences. It also illustrates the need to develop diagnostic tests that are sensitive in local breeds.
Assuntos
Vírus da Artrite-Encefalite Caprina/genética , Infecções por Lentivirus/epidemiologia , Vírus Visna-Maedi/genética , Visna/epidemiologia , Sequência de Aminoácidos , Animais , Produtos do Gene gag/genética , Genes pol/genética , Genoma Viral , Cabras , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Ovinos , Espanha/epidemiologia , Sequências Repetidas Terminais/genéticaRESUMO
A three year long experimental study was carried out to investigate horizontal MVV-infection by PCR and ELISA, in 191 one year-old latxa dairy-sheep raised in two separate groups under low and high MVV-infection pressure, respectively. Sheep originated from a previous MVV-transmission study in lambs and seroprevalence among one year-old sheep in both groups was 15% approximately. The high infection-pressure group (H-group) consisted of 147 replacement ewes that joined a milk-producing, housed dairy-flock with 42-66% MVV-seroprevalence and the low infection-pressure group (L-group) were castrated males raised in a separate shed. In contrast to results obtained when infection was investigated in lambs, the overall degree of agreement between ELISA and PCR results was very good and there was some indication that it increased further as sheep became older. MVV-prevalence did not change in the L-group and increased to 57% in three year-old sheep in the H-group (p<0.001). Random effects logistic regression confirmed seroconversion was significantly higher in the H-group compared to the L-group and was highest during the year after the sheep were introduced in the dairy flock and did not increase with age as in previous studies using less sensitive antibody assays. The evidence that horizontal transmission can be very low in spite of prolonged close contact between infected and non-infected sheep is valuable for MVV-control purposes. Furthermore it highlights the need to investigate virus excretion dynamics in infected animals and animal to animal transmission to improve our overall understanding of horizontal MVV transmission in MVV endemic populations.
Assuntos
Transmissão de Doença Infecciosa/veterinária , Pneumonia Intersticial Progressiva dos Ovinos/transmissão , Vírus Visna-Maedi/isolamento & purificação , Envelhecimento , Animais , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Razão de Chances , Pneumonia Intersticial Progressiva dos Ovinos/imunologia , Pneumonia Intersticial Progressiva dos Ovinos/virologia , Reação em Cadeia da Polimerase/veterinária , Ovinos , Vírus Visna-Maedi/genética , Vírus Visna-Maedi/imunologiaRESUMO
A recent large-scale experimental study showed that bottle-feeding ovine colostrum from seropositive ewes results in high MVV-seroconversion in lambs. In contrast, relatively few lambs that naturally suckled colostrum from seropositive dams seroconverted as a result of it. Furthermore, lambs fed uninfected bovine colostrum readily seroconverted when mixed with ovine-colostrum lambs indicating that horizontal MVV transmission between lambs was efficient. MVV-infection was further investigated in the same samples using two PCR tests targeting sequences in the long-terminal repeats (LTR) and POL MVV genes. PCR-tests confirmed previous serological findings. However, the LTR-PCR was more sensitive and allowed detecting infection earlier than the other tests, including 5-8% of new-born lambs from seropositive dams, providing more evidence that prenatal MVV-infection may be more important than considered. The degree of agreement between PCR and antibody tests in individual samples was low up to 6 months of age and moderate at 10 months-old. Nine percent of lambs were always PCR-negative but seroconverted and 19% of lambs were PCR-positive at least once and did not seroconvert. However, seroconversion was associated with increasing number of times lambs were PCR-positive and ovine colostrum-fed lambs were more frequently PCR-positive than other lambs. The significance of these findings in terms of MVV-infection, epidemiology and control is discussed.
Assuntos
Anticorpos Antivirais/sangue , Colostro/virologia , Transmissão Vertical de Doenças Infecciosas/veterinária , Pneumonia Intersticial Progressiva dos Ovinos/imunologia , Pneumonia Intersticial Progressiva dos Ovinos/transmissão , Vírus Visna-Maedi/genética , Vírus Visna-Maedi/isolamento & purificação , Envelhecimento , Animais , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Razão de Chances , Pneumonia Intersticial Progressiva dos Ovinos/sangue , Pneumonia Intersticial Progressiva dos Ovinos/virologia , Reação em Cadeia da Polimerase/veterinária , OvinosRESUMO
Maedi visna virus and caprine arthritis encephalitis virus are closely related retroviruses that cause chronic inflammatory disease in small ruminants. The infections are characterised by insidious onset and slow progression. Diagnosis of infection is usually by serological testing. A variety of assays are available for this purpose, though the relative sensitivity and specificity of these assays has not been compared systematically. Here we review recent developments in laboratory diagnostic methods and their use in field diagnosis. The results suggest that a combination of ELISA and PCR might afford optimal detection of SRLV infection.
Assuntos
Vírus da Artrite-Encefalite Caprina/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/veterinária , Infecções por Lentivirus/veterinária , Reação em Cadeia da Polimerase/veterinária , Ruminantes/virologia , Vírus Visna-Maedi/isolamento & purificação , Animais , Vírus da Artrite-Encefalite Caprina/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Doenças das Cabras/diagnóstico , Cabras , Imunodifusão/métodos , Imunodifusão/veterinária , Infecções por Lentivirus/diagnóstico , Pneumonia Intersticial Progressiva dos Ovinos/diagnóstico , Reação em Cadeia da Polimerase/métodos , Sensibilidade e Especificidade , Testes Sorológicos/métodos , Testes Sorológicos/veterinária , Ovinos , Doenças dos Ovinos/diagnóstico , Vírus Visna-Maedi/imunologiaRESUMO
Maedi-visna virus (MVV) seroprevalence associated with consumption of colostrum from seropositive ewes was investigated in 276 housed lambs from birth to 300 days-old. At birth, lambs were allocated to five experimental groups according to the maternal MVV-serological status, source and mode of feeding colostrum (bovine or ovine and bottle fed or suckled from the dam) and type of horizontal MVV-exposure (raised with the dam or separately with other lambs). The risk of being seropositive at 300 days-old was associated with feeding ovine colostrum from seropositive ewes and increased with intake of bottle-fed ovine colostrum and was higher in lambs separated from their dams and raised with other experimental lambs compared to lambs raised with their dams. Approximately 75-87% of ELISA-positive results in lambs that had ovine colostrum was attributable to colostrum itself. However, approximately only 16% of naturally raised and 29-61% of bottle-fed ovine colostrum lambs were ELISA-positive as a result feeding ovine colostrum. These results confirm that ovine colostrum from seropositive ewes can be a major source of MVV but its overall contribution to seroprevalence in natural conditions is relatively low, and shows that horizontal MVV transmission can be an important source of infection in new-born lambs.
Assuntos
Colostro/virologia , Transmissão Vertical de Doenças Infecciosas/veterinária , Pneumonia Intersticial Progressiva dos Ovinos/transmissão , Doenças dos Ovinos/transmissão , Vírus Visna-Maedi , Envelhecimento , Animais , Anticorpos Antivirais/sangue , Feminino , Estudos Longitudinais , Risco , Estudos Soroepidemiológicos , Ovinos , Vírus Visna-Maedi/imunologiaRESUMO
Small ruminant lentiviruses include viruses with diverse genotypes that frequently cross the species barrier between sheep and goats and that display a great genetic variability. These characteristics stress the need to consider the whole host range and to perform local surveillance of the viruses to opt for optimum diagnostic tests, in order to establish control programmes. In the absence of effective vaccines, a comprehensive knowledge of the epidemiology of these infections is of major importance to limit their spread. This article intends to cover these aspects and to summarise information related to characteristics of the viruses, pathogenesis of the infection and description of the various syndromes produced, as well as the diagnostic tools available, the mechanisms involved in transmission of the pathogens and, finally, the control strategies that have been designed until now, with remarks on the drawbacks and the advantages of each one. We conclude that there are many variables influencing the expected cost and benefits of control programs that must be evaluated, in order to put into practice measures that might lead to control of these infections.
Assuntos
Infecções por Lentivirus/veterinária , Lentivirus/genética , Ruminantes/virologia , Doenças dos Ovinos/diagnóstico , Animais , Doenças das Cabras/diagnóstico , Doenças das Cabras/etiologia , Doenças das Cabras/prevenção & controle , Cabras , Especificidade de Hospedeiro , Interações Hospedeiro-Patógeno , Lentivirus/fisiologia , Infecções por Lentivirus/diagnóstico , Infecções por Lentivirus/etiologia , Infecções por Lentivirus/prevenção & controle , Ovinos , Doenças dos Ovinos/etiologia , Doenças dos Ovinos/prevenção & controle , Carneiro DomésticoRESUMO
Small ruminant lentiviruses (SRLV) are classical slow retroviruses causing chronic inflammatory disease in a variety of target organs. The routes of transmission have been investigated and a large body of evidence has accumulated over many years. The main routes are through ingestion of infected colostrum and/or milk, or through inhalation of respiratory secretions. However, many studies also provide evidence that intrauterine infection may occur, though the extent and significance of this route is controversial. Embryos treated to IETS standards appear to pose very little risk of infection. SRLV have been detected in semen suggesting a potential source of transmission. However, such transmission has not been demonstrated to date. The application of control measures based on this information allows more efficient strategies to be developed which will reduce the rate of transmission.
Assuntos
Doenças das Cabras/transmissão , Doenças das Cabras/virologia , Infecções por Lentivirus/veterinária , Lentivirus Ovinos-Caprinos/crescimento & desenvolvimento , Doenças dos Ovinos/transmissão , Doenças dos Ovinos/virologia , Animais , Transmissão de Doença Infecciosa/veterinária , Cabras , Transmissão Vertical de Doenças Infecciosas/veterinária , Infecções por Lentivirus/transmissão , Infecções por Lentivirus/virologia , OvinosRESUMO
A total of 14 antibodies were found to label resting and/or activated swine platelets. Six recognized CD previously characterized for swine (CD29, CD41/61 and CD46). One had been characterized for human cells (CD47). Two antibodies with CD14 and SLA class I specificity suggested by the donor as well as five blind antibodies were also positive on platelets. One antibody appeared to recognize the swine homologue to human CD47, and four remained unclustered.
Assuntos
Plaquetas/imunologia , Suínos/imunologia , Animais , Anticorpos Monoclonais , Especificidade de Anticorpos , Antígenos CD , Citometria de Fluxo , Humanos , Especificidade da EspécieRESUMO
The genetic variability within the Uruguayan Creole horse and its relationship to a group of geographically or historically related breeds (Spanish Pure-bred, Barb, Quarter horse, Paso Fino, Peruvian Paso, Arabian and Thoroughbred horse), was evaluated using 25 loci (seven of blood groups, nine of protein polymorphisms and nine microsatellites) analyzed on a total of 145 Uruguayan Creole horses. In this study, blood group and protein polymorphism variants that are considered to be breed markers of Spanish Pure-bred and Barb horses were detected in the Creole breed. Conversely, some microsatellites and protein polymorphisms alleles were found uniquely in the Creole horse. American horse breeds together with Barb and Arabian horses clearly formed a separate cluster from the Spanish pure-bred and Thoroughbred breeds, as shown by an UPGMA dendrogram based on Nei's standard genetic distance. Data in this study provided evidence for considerable genetic variation within Uruguayan Creole horses and of a distinctive breed profile. Both traits were most likely inherited from the XVIth century Spanish horses, more closely related to Barb than to Spanish Pure-bred.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Cavalos/genética , Repetições de Microssatélites/genética , Filogenia , Polimorfismo Genético/genética , Animais , Frequência do Gene , Variação Genética/genética , Cavalos/classificação , UruguaiAssuntos
Anestésicos Dissociativos/administração & dosagem , Anestésicos Intravenosos/administração & dosagem , Ketamina/administração & dosagem , Piperidinas/administração & dosagem , Timpanoplastia , Anestésicos Intravenosos/farmacocinética , Criança , Contraindicações , Humanos , Infusões Intravenosas , Injeções Intravenosas , Masculino , Óxido Nitroso , Piperidinas/farmacocinética , RemifentanilRESUMO
A control system for Visna/maedi virus (VMV) infection based on serologic segregation and management strategies was applied in an infected Spanish dairy Manchega breed sheep flock (n=670) that was affected by a severe respiratory process associated to VMV. The control started in 2004 and consisted on the serological study of animals, segregation in two different flocks (seropositive and seronegative), separate management of flocks, selection of young female lambs for replacement only from seronegative ewes offspring, immediate removal of seropositive animals detected in the seronegative flock and a management tending toward the reduction and final culling of the seropositive flock. The serological control was repeated yearly or twice a year, approximately. Initial VMV seroprevalence of the undivided flock was 66.4% (January 2004) that descended to 47.3%, 12.8%, 2.2% and 0.2% between July 2004 and May 2006. Residual seroprevalence fluctuated slightly thereafter with a peak of 2.2% in April 2008. After segregation, number of animals in the seronegative flock was 378 that descended to 323 in October 2005. Since then, this number has increased steadily reaching 650 sheep in December 2011. The seropositive flock was progressively reduced by culling until its total disappearance in June 2010. This work presents the detailed results obtained in the control strategy against VMV in a single dairy sheep flock by implementing a segregation system based on serologic testing. The system is highly successful, as it reduces to residual levels VMV infection in about two years without the need of culling a high number of animals, as required by other methods. Moreover, the original size flock was been recovered within 8 years and has led to a subjective improvement of animal health and welfare in the flock. The residual seroprevalence could be eliminated at this stage by applying more sensitive molecular or other serological techniques to reach eradication.
Assuntos
Indústria de Laticínios/métodos , Pneumonia Intersticial Progressiva dos Ovinos/prevenção & controle , Vírus Visna-Maedi/fisiologia , Visna/prevenção & controle , Animais , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Pneumonia Intersticial Progressiva dos Ovinos/virologia , Prevalência , Estudos Soroepidemiológicos , Ovinos , Espanha/epidemiologia , Visna/virologiaRESUMO
A single broadly reactive standard ELISA is commonly applied to control small ruminant lentivirus (SRLV) spread, but type specific ELISA strategies are gaining interest in areas with highly prevalent and heterogeneous SRLV infections. Short (15-residue) synthetic peptides (n=60) were designed in this study using deduced amino acid sequence profiles of SRLV circulating in sheep from North Central Spain and SRLV described previously. The corresponding ELISAs and two standard ELISAs were employed to analyze sera from sheep flocks either controlled or infected with different SRLV genotypes. Two outbreaks, showing SRLV-induced arthritis (genotype B2) and encephalitis (genotype A), were represented among the infected flocks. The ELISA results revealed that none of the assays detected all the infected animals in the global population analyzed, the assay performance varying according to the genetic type of the strain circulating in the area and the test antigen. Five of the six highly reactive (57-62%) single peptide ELISAs were further assessed, revealing that the ELISA based on peptide 98M (type A ENV-SU5, consensus from the neurological outbreak) detected positives in the majority of the type-A specific sera tested (Se: 86%; Sp: 98%) and not in the arthritic type B outbreak. ENV-TM ELISAs based on peptides 126M1 (Se: 82%; Sp: 95%) and 126M2 0,65 0.77 (Se: 68%; Sp: 88%) detected preferentially caprine arthritis encephalitis (CAEV, type B) and visna/maedi (VMV, type A) virus infections respectively, which may help to perform a preliminary CAEV vs. VMV-like typing of the flock. The use of particular peptide ELISAs and standard tests individually or combined may be useful in the different areas under study, to determine disease progression, diagnose/type infection and prevent its spread.
Assuntos
Ensaio de Imunoadsorção Enzimática/veterinária , Infecções por Lentivirus/veterinária , Doenças dos Ovinos/diagnóstico , Sequência de Aminoácidos , Animais , Anticorpos Antivirais/sangue , Antígenos Virais/genética , Vírus da Artrite-Encefalite Caprina/genética , Vírus da Artrite-Encefalite Caprina/imunologia , Surtos de Doenças/veterinária , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/estatística & dados numéricos , Genes gag , Cabras , Infecções por Lentivirus/diagnóstico , Infecções por Lentivirus/epidemiologia , Dados de Sequência Molecular , Filogenia , Pneumonia Intersticial Progressiva dos Ovinos/diagnóstico , Pneumonia Intersticial Progressiva dos Ovinos/epidemiologia , Pneumonia Intersticial Progressiva dos Ovinos/imunologia , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/imunologia , Carneiro Doméstico , Espanha/epidemiologia , Proteínas Virais/genética , Proteínas Virais/imunologia , Visna/diagnóstico , Visna/epidemiologia , Visna/imunologia , Vírus Visna-Maedi/genética , Vírus Visna-Maedi/imunologiaRESUMO
An extensive outbreak characterized by the appearance of neurological symptoms in small ruminant lentivirus (SRLV) infected sheep has been identified in Spain, but the genetic characteristics of the strain involved and differential diagnostic tools for this outbreak remain unexplored. In this work, 23 Visna-affected naturally infected animals from the outbreak, 11 arthritic animals (both groups presenting anti-Visna/Maedi virus serum antibodies), and 100 seronegative animals were used. Eight of the Visna-affected animals were further studied post-mortem by immunohistochemistry. All had lesions in spinal cord, being the most affected part of the central nervous system in six of them. A representative strain of the outbreak was isolated. Together with other proviral sequences from the outbreak the virus was assigned to genotype A2/A3. In vitro culture of the isolate revealed that viral production was slow/low in fibroblast-like cells but it was high in blood monocyte-derived macrophages. The long terminal repeat (LTR) of the viral genome of this isolate lacked an U3-duplication, but its promoter activity in fibroblast-like cells was normal compared to other strains. Thus, viral production could not be inferred from the LTR promoter activity in this isolate. Analysis of the viral immunodominant epitopes among SRLV sequences of the outbreak and other known sequences allowed the design of a synthetic SU peptide ELISA that detected the Visna affected animals, representing a tool of epidemiological interest to control viral spread of this highly pathogenic strain.
Assuntos
Surtos de Doenças/veterinária , Vírus Visna-Maedi/genética , Visna/diagnóstico , Visna/virologia , Animais , Anticorpos Antivirais/sangue , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Macrófagos/virologia , Masculino , Ovinos , Carneiro Doméstico , Espanha/epidemiologia , Sequências Repetidas Terminais , Visna/epidemiologia , Vírus Visna-Maedi/imunologia , Vírus Visna-Maedi/isolamento & purificaçãoRESUMO
Nucleotide sequences of small ruminant lentiviruses (SRLVs) were determined in sheep and goats, including progeny of imported animals, on a farm in Mexico. On the basis of gag-pol, pol, env and LTR sequences, SRLVs were assigned to the B1 subgroup, which comprises caprine arthritis-encephalitis virus (CAEV)-like prototype sequences mainly from goats. In comparison with CAEV-like env sequences of American and French origin, two putative recombination events were identified within the V3-V4 and V4-V5 regions of the env gene of a full length SRLV sequence (FESC-752) derived from a goat on the farm.
Assuntos
Vírus da Artrite-Encefalite Caprina/classificação , Vírus da Artrite-Encefalite Caprina/genética , Genes Virais , Doenças das Cabras/virologia , Infecções por Lentivirus/veterinária , Recombinação Genética , Doenças dos Ovinos/virologia , Animais , Sequência de Bases , Genes env , Genes gag , Genes pol , Cabras , Infecções por Lentivirus/virologia , México , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Ovinos , Carneiro Doméstico , Sequências Repetidas TerminaisRESUMO
The incidence of seroconversion to visna/maedi virus (VMV) infection and its relationship with management and sheep building structure was investigated in 15 dairy sheep flocks in Spain during 3-7years. Incidence rates were 0.09 per sheep-year at risk in semi-intensive Latxa flocks and 0.44 per sheep-year at risk in intensive Assaf flocks and was greatest for the one year old Assaf replacement flock. Separate multivariable models developed for replacement and adult flocks indicated that in both cases seroconversion was strongly associated to direct contact exposure to infected sheep and to being born to a seropositive dam. The latter effect was independent of the mode of rearing preweaning and the risk of seroconversion was similar for sheep fed colostrum and milk from a seropositive or a seronegative dam. These results are further evidence of the efficiency of horizontal VMV transmission by close contact between sheep and also suggest a inheritable component of susceptibility and resistance to infection. In contrast, indirect aerogenous contact with seropositive sheep was not associated with seroconversion as evidenced in replacement sheep housed in separate pens in the same building as adult infected sheep for one year. Consequently, VMV may not be efficiently airborne over short distances and this is important for control of infection. Moreover, there was no relationship between seroconversion and shed open areas. The latter could be related to having examined few flocks in which high infection prevalence dominated the transmission process while ventilation, may depend on a variety of unrecorded factors whose relationship to infection needs to be further investigated.
Assuntos
Abrigo para Animais/normas , Pneumonia Intersticial Progressiva dos Ovinos/epidemiologia , Doenças dos Ovinos/epidemiologia , Vírus Visna-Maedi/isolamento & purificação , Visna/epidemiologia , Envelhecimento , Animais , Anticorpos Antivirais/sangue , Cruzamento/normas , Colostro/virologia , Indústria de Laticínios/normas , Feminino , Incidência , Leite/virologia , Pneumonia Intersticial Progressiva dos Ovinos/sangue , Pneumonia Intersticial Progressiva dos Ovinos/prevenção & controle , Ovinos , Doenças dos Ovinos/prevenção & controle , Doenças dos Ovinos/virologia , Espanha/epidemiologia , Visna/sangue , Visna/prevenção & controleRESUMO
A serological survey of Visna/maedi virus (VMV) infection involving 274,048 sheep from 554 flocks was undertaken during 2002-2007 in Aragón, North-East Spain. One hundred and two of these flocks enrolled in a VMV control programme to reduce seroprevalence by selecting replacement lambs from seronegative dams and gradual culling of seropositive sheep. Twenty-five flocks were also visited to collect flock management and housing data. All study flocks had seropositive animals and 52.8% of animals tested were seropositive. Among flocks that joined the control programme 66 adopted the proposed measures and reduced seroprevalence significantly by between 26.1% and 76.9% whereas the remaining 36 flocks did not apply the measures and seroprevalence significantly increased. Seroprevalence increased with flock size and the number of days the sheep were housed, and decreased with increasing weaning age and shed open area, suggesting a reduced risk of VMV infection in sheep associated with better ventilation. At the end of the period, 24 flocks were certified as VMV-controlled with a seroprevalence <5%, and seven as VMV-free with 0% seroprevalence. These are the first officially recognised VMV-free flocks in Spain and represent a nucleus of VMV-free replacement animals for other flocks. Moreover, they are evidence of the possibility of eliminating VMV infection without resorting to whole-flock segregation or culling of seropositive sheep.