Assessment of microbial flora and pesticidal effect of vermicast generated from Azadirachta indica (neem) for developing a biofertilizer-cum-pesticide as a single package.

The soil comprising organic matter, nutrients, serve as substrate for plant growth and various organisms. In areas where there are large plantations, there is a huge leaf litter fall. The leaf litter upon decomposition releases nutrients and helps in nutrient recycling, for which the soil engineers such as earthworms, ants and termites are important key players. In this context, the present study was conducted to assess the characteristics of the vermicast obtained by vermicomposting neem leaf litter in terms of microbial flora, plant growth promoting properties and antagonistic activities of the vermicast against phytopathogens. Vermicomposting of neem leaf litter was done using two epigeic earthworm species Eisenia fetida and Eudrilus eugeniae. The vermicast exhibited antagonistic potential against plant pathogens. Out of the four vermiwash infusions studied, the 75 % formulation reduced the disease incidence against mealybug by 82 % in the tree Neolamarkia cadamba. The result of the study suggests that vermicast made from neem leaf litter may be a potent combination of a biofertilizer and a pesticide.

Investigation on the physico-chemical properties of soil and mineralization of three selected tropical tree leaf litter.

Plant leaf litter has a major role in the structure and function of soil ecosystems as it is associated with nutrient release and cycling. The present study is aimed to understand how well the decomposing leaf litter kept soil organic carbon and nitrogen levels stable during an incubation experiment that was carried out in a lab setting under controlled conditions and the results were compared to those from a natural plantation. In natural site soil samples, Anacardium. occidentale showed a higher value of organic carbon at surface (1.14%) and subsurface (0.93%) and Azadirachta. indica exhibited a higher value of total nitrogen at surface (0.28%) and subsurface sample (0.14%). In the incubation experiment, Acacia auriculiformis had the highest organic carbon content initially (5.26%), whereas A. occidentale had the highest nitrogen level on 30th day (0.67%). The overall carbon-nitrogen ratio showed a varied tendency, which may be due to dynamic changes in the complex decomposition cycle. The higher rate of mass loss and decay was observed in A. indica leaf litter, the range of the decay constant is 1.26-2.22. The morphological and chemical changes of soil sample and the vermicast were substantained using scanning electron microscopy (SEM) and Fourier transmission infrared spectroscopy (FT-IR).

Vermitechnology transforms hazardous red mud into benign organic input for agriculture: Insights on earthworm-microbe interaction, metal removal, and soil-crop improvement.

Bioremediation of hazardous bauxite residues, red mud (RM), through vermicomposting has yet to be attempted. Therefore, the valorization potential of Eisenia fetida in various RM and cow dung (CD) mixtures was compared to aerobic composting. Earthworm fecundity and biomass growth were hindered in RM + CD (1:1) feedstock but enhanced in RM + CD (1:3). The pH of highly alkaline RM-feedstocks sharply reduced (>17%) due to vermicomposting. N, P, and K availability increased dramatically with Ca and Na reduction under vermicomposting. Additionally, ∼40-60% bioavailable metal fractions were transformed to obstinate (organic matter and residual bound) forms upon vermicomposting. Consequently, the total metal concentrations were significantly reduced with considerably high earthworm bioaccumulation. Microbial growth and enzyme activity were more significant under vermicomposting than composting. Correlation statistics revealed that microbial augmentation significantly facilitated a metal reduction in RM-vermibeds. Eventually, RM-vermicompost stimulated sesame growth and improved soil health with the least heavy metal contamination to soil and crop.

Effect of conventional and biodegradable microplastics on earthworms during vermicomposting process.

The potential effect of microplastics is an increasingly growing environmental issue. However, very little is known regarding the impact of microplastics on the vermicomposting process. The present study explored the effect of non-biodegradable (low density polyethylene; LDPE) and biodegradable (polybutylene succinate-co-adipate; PBSA) microplastics on earthworm Eisenia fetida during vermicomposting of cow dung. For this, earthworms were exposed to different concentrations (0, 0.5, 1 and 2%) of LDPE and PBSA of 2 mm size. The cow dung supported the growth and hatchlings of earthworms, and the toxicity effect of both LDPE and PBSA microplastics on Eisenia fetida was analyzed. Microplastics decreased the body weight of earthworms and there was no impact on hatchlings. The body weight of earthworm decreased from 0 to 60th day by 18.18% in 0.5% of LDPE treatment, 5.42% in 1% of LDPE, 20.58% in 2% of LDPE, 19.99% in 0.5% of PBSA, 15.09% in 1% of PBSA and 16.36% in 2% of PBSA. The physico-chemical parameters [pH (8.55-8.66), electrical conductivity (0.93-1.02 (S/m), organic matter (77.6-75.8%), total nitrogen (3.95-4.25 mg/kg) and total phosphorus (1.16-1.22 mg/kg)] do not show much significant changes with varying microplastics concentrations. Results of SEM and FTIR-ATR analysis observed the surface damage of earthworms, morphological and biochemical changes at higher concentrations of both LDPE and PBSA. The findings of the present study contribute to a better understanding of microplastics in vermicomposting system.

Understanding vermicompost and organic manure interactions: impact on toxic elements, nitrification activity, comammox Nitrospira inopinata, and archaea/bacteria.

Vermicompost is a substantial source of nutrients, promotes soil fertility, and maintains or increases soil organic matter levels. Potentially toxic elements (PTEs) in vermicompost impact on nitrification activity. However, it is yet unknown how vermicompost affects nitrifying bacteria and archaea, comammox Nitrospira inopinata (complete ammonia oxidizers), net nitrification rates (NNRs), and PTEs. The effects of vermicompost application on NNRs, potential nitrification rates (NPs), PTEs, and the abundances of comammox N. inopinata bacteria, nitrite-oxidizing bacteria (NOB), and ammonia-oxidizing bacteria (AOB)/archaea (AOA) were studied. NNRs and NPs were significantly higher (p < 0.05) in fresh cow-dung vermicompost (stored for 40 days) as compared with other organic manure. The level of PTEs (Cu2+, Fe2+, Pb2+, Cd2+, and Zn2+) was significantly lower (p < 0.05) in vermicompost as compared with compost of waste material with Trichoderma and cow dung. Comammox N. inopinata, NOB, AOB, and AOA were significantly higher (p < 0.05) in stored cow-dung vermicompost (more than 1 year) as compared with other organic manure. The results of the scatterplot matrix analysis suggested that Fe2+, total nitrogen (TN), soil organic carbon (SOC), and total carbon (TC) were linearly correlated (p < 0.001) with NNRs and NPs in vermicompost and organic manure. Similarly, comammox N. inopinata bacteria, NOB, AOB, and AOA were linearly correlated (p < 0.001) with NNR and NP. These results indicated that vermicompost promoted nitrification activity by increasing microbial diversity and abundance, supplying nutrients and organic matter for microbial growth, and facilitating complex microbial interactions. It may be concluded that the influence of vermicompost, which played a great role in PTE concentration reduction, increased chemical, and biological properties, increased the growth rate of nitrifying bacteria/archaea and the nitrogen cycle.

Bacillus subtilis reduces antibiotic resistance genes of animal sludge in vermicomposting by improving heat stress tolerance of Eisenia foetida and bacterial community adjustment.

Antibiotic resistance genes (ARGs) in livestock industry have been recognized as a kind of pollutant. The effect of Bacillus subtilis (B. subtilis) as an additive for the reduction of ARGs in animal sludge from livestock and poultry wastewater treatment plant during vermicomposting was investigated. We also evaluated the oxidative stress level and growth of earthworms, Eisenia foetida, bacterial community succession, and the quality of the end products. Two treatments were conducted using B. subtilis, one at 18 °C and another at 28 °C. Controls were setup without the bacteria. The results showed that inoculation of B. subtilis promoted the degradation of organics at 28 °C and increased the germination index to 236%. The increased activities of the superoxide dismutase (1.69 U/mg pr) and catalase (8.05 U/mg pr) and the decreased activity of malondialdehyde (0.02 nmol/mg pr) by B. subtilis at 28 °C showed that the earthworms were relieved of heat stress. The addition of B. subtilis reduced the abundance of 32 target ARGs, including integron (intI-1), transposase (IS613) and resistant genes, such as sulfonamide (sul2), quinolone (oprJ), macrolide-lincosamide-streptogramin group B (ermF, ermB), tetracycline (tetL-02, tetX), ß-lactama (blaOXA10-01) and aminoglycoside [strB, aac(6')-Ib(aka aacA4)-01, aac(6')-Ib(aka aacA4)-02]. Organic matter degrading Membranicola, Paludisphaera, Sphingorhabdus and uncultured bacterium belonging to the order Chitinophagales, nitrifying and nitrogen-fixing Singulisphaera and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, soil remediating Achromobacter, and plant growth promoting Kaistia, Galbibacter and Ilumatobacter were increased significantly (P < 0.05). However, the growth of harmful bacteria such as Burkholderiaceae was inhibited in the vermicompost. In earthworm guts, the probiotic Mesorhizobium was promoted, while the pathogenic uncultured bacterium belonging to the family Enterobacteriaceae was reduced. Besides, B. subtilis enhanced the host relationships between bacteria and ARGs. These findings might be helpful in the removal of ARGs in animal wastes and in understanding the synergy between earthworms and microorganisms.

Production and characterization of enriched vermicompost from banana leaf biomass waste activated by biochar integration.

Vermicomposting uses less energy and requires fewer infrastructures, and it is capable of restoring soil nutrition and carbon. Banana cultivation produces lots of trash in a single crop season, with 30 tonnes of waste generated per acre. The biodegradable fraction of banana leaf waste is thrown out in large quantities from temples, markets place wedding halls, hotels, and residential areas. Vermicomposting can be used for recovering lignin, cellulose, pectin, and hemicellulose from banana leaves. Earthworm digests organic materials with the enzymes produced in gut microflora. Biochar adds bulk to vermicomposting, increases its value as fertilizer. The goal of this study was to amend biochar (0, 2, 4 and 6%) with banana leaf waste (BLW) + cow dung (CD) in three different combinations (1:1, 2:1 and 3:1) using Eisenia fetida to produce enriched vermicompost. In the vermicompost with biochar groups, there were higher levels of physicochemical parameters, as well as macro- and micronutrient contents. The growth and reproduction of earthworms were higher in groups with biochar. A maximum of 1.82, 1.18 and 1.67% of total nitrogen, total phosphorus and total potassium was found in the final vermicompost recovered from BLW + CD (1:1) amended with 4% biochar; while the other treatments showed lower levels of nutrients. A lower C/N ratio of 18.14 was observed in BLW + CD (1:1) + 4% biochar followed by BLW + CD (1:1) + 2% biochar amendment (19.92). The FTIR and humification index studies show that degradation of organic matter has occurred in the final vermicompost and the substrates with 4% biochar in 1:1 combination showed better degradation and this combination can be used for nutrient rich vermicompost production.

Bioconversion of fruit waste and sewage sludge mixtures by black soldier fly (Diptera: Stratiomyidae) larvae.

Bioconversion of fruit waste (FW) and sewage sludge (SS) sludge mixtures into valuable products was investigated using black soldier fly (Hermetia illucens) larvae (BSFL) under a lab-scale trial. For that, five different setups of FW and SS mixtures (100FW; 100SS; 70SS+30FW; 50SS+50FW; 70FW+30SS) were prepared and changes in larval biomass, feed loss, and residual waste physicochemical properties were estimated until the emergence of fly in all waste mixtures. BSFL caused a significant decrease in total organic carbon (11.71-34.79%) and carbon-to-nitrogen ratio (C/N ratio) while the increase in total nitrogen (8.35-123.30%), total phosphorus (17.02-143.36%), and total potassium (19.40-48.87%) contents in the feedstock. The germination index and C/N ratio of frass were below the standards decided for manure quality in a few setups suggesting the non-stability of frass for agronomic applications due to the short duration (20 d) of composting. Larval biomass yield, feed conversion ratio and nutrient mineralization were found to be higher in 50SS+50FW and 70FW+30SS feedstock combinations suggesting their suitability as ideal feedstock for optimal BSFL cultivation. The impact of toxic substances in sewage on BSFL survival, growth and waste stabilization processes, and frass metal enrichment could be investigated in future studies.

From waste to wealth: Innovations in organic solid waste composting.

Organic solid waste (OSW) is not only a major source of environmental contamination, but also a vast store of useful materials due to its high concentration of biodegradable components that can be recycled. Composting has been proposed as an effective strategy for recycling OSW back into the soil in light of the necessity of a sustainable and circular economy. In addition, unconventional composting methods such as membrane-covered aerobic composting and vermicomposting have been reported more effective than traditional composting in improving soil biodiversity and promoting plant growth. This review investigates the current advancements and potential trends of using widely available OSW to produce fertilizers. At the same time, this review highlights the crucial role of additives such as microbial agents and biochar in the control of harmful substances in composting. Composting of OSW should include a complete strategy and a methodical way of thinking that can allow product development and decision optimization through interdisciplinary integration and data-driven methodologies. Future research will likely concentrate on the potential in controlling emerging pollutants, evolution of microbial communities, biochemical composition conversion, and the micro properties of different gases and membranes. Additionally, screening of functional bacteria with stable performance and exploration of advanced analytical methods for compost products are important for understanding the intrinsic mechanisms of pollutant degradation.

Composting and vermicomposting of sewage sludge at various C/N ratios: Technological feasibility and end-product quality.

Even though sewage sludge (SS) contains a high level of pollutants, it is rich in essential plant nutrients and has the potential to enhance soil fertility. However, the SS must be further treated through pre-composting plus vermicomposting to make it safe for use on food crops. More research and data are needed to determine how different carbon-to-nitrogen ratios (C/N) affect the feasibility and quality of composting vs vermicomposting of SS. Therefore, in this study we comprehensively evaluated the feasibility and end-product quality of compost and vermicompost produced from SS under different C/N ratios. SS was mixed with pelletized wheat straw (PWS) at various proportions to produce C/N ratios of 6:1, 18:1, 28:1, and 38:1, then pre-composted for 14 days followed by vermicomposting using the earthworm Eisenia andrei for 120 days. Agrochemical properties were measured at 0, 30, 60, 90, and 120 days. Results revealed significantly higher levels of agrochemicals in vermicompost compared to compost, including total potassium (37-88%) and magnesium (4.3-12%), nitrate nitrogen (71-98%), available potassium (53-88%), available phosphorus (79%), available magnesium (54-453%), available boron (48-303%), and available copper (2.5-82%). However, lower levels of ammonium nitrogen by (59-85%), available iron (2.3-51.3%), available manganese (29.7-52.2%), available zinc (10.5-29.8%), total carbon (0.75-4.5%), and total nitrogen (1.6-22.2%) were measured. Comparison of the various C/N ratios, showed that vermicompost with an 18:1 C/N ratio outperformed compost and demonstrated the highest earthworm population (165 pieces/kg). Thus, vermicomposting SS at an 18:1 C/N ratio is strongly recommended as a sustainable technology for producing high-quality vermicompost from SS.

Synthesizing biochar-based slow-releasing fertilizers using vermicompost leachate, cow dung, and plant weed biomass.

Biochar-based slow-releasing fertilizers (BSRF) have been recommended widely for efficient soil nutrient management and crop production. In this study, we examined the N, P, and K release behaviour of pyrolysed (at 350 °C) cow dung (CDB), vermicompost (VCB), and Lantana (LB) weed and impregnated LB (LBVW) and CDB (CDBVW) with vermicompost leachate (1:1 v/v) under a lab-scale trial. BSRFs (CDB, VCB, LBVW and VCBVW) characterization (FT-IR, SEM-EDX and surface area analysis) was done and then tested for its suitability for soil-plant applications. Soil incubation study indicated the slow-releasing behaviour of BSRFs and overall P, N, and K release was found to be in the ranges of 72.3-84.5%, 73.1-79.0%, and 43.1-85.3%, respectively in different BSRFs setups. Furthermore, lab trials suggested the highest P (64.5%), N (75.3%), and K (86.8%) uptakes by the plant (Vigna radiata) in CDBVW and LBVW setups. Moreover, pot trails with moong bean (Vigna radiata) suggested a high growth in shoot and root and plant yield as well in seedlings cultivated with BSRFs. This study indicates that animal manure, vermicompost and terrestrial weed Lantana biochar can be used effectively to prepare BSRFs for efficient soil-plant nutrient management with multiple environmental benefits.

Biochar improves compost humification, maturity and mitigates nitrogen loss during the vermicomposting of cattle manure-maize straw.

Maintaining humidification and inhibiting nitrogen losses during vermicomposting process have emerged to be key factors for high-quality productions. Previous data have showed outstanding functions of biochar addition in improving vermicomposting quality. In this study, the influence of bamboo biochar (BB) and rice husk biochar (RHB) addition on compost maturity, humification and nitrogen loss was evaluated in the vermicomposting of cattle manure and maize straw. Results revealed that BB or RHB amendment improved organic matter decomposition, enhanced humification and maturity of compost, particularly in the 10% BB treatment, which exerted the highest humic acids content and GI value. Furthermore, BB or RHB addition significantly reduced nitrogen losses, in which the volatilization of NH3 and N2O were reduced by 24.93%-66.23% and 14.91%-55.12%. The fewest nitrogen loss was detected in the treatment of 10% BB. Biochar inhibited nirK, nirS but promoted AOB-amoA, nosZ expression; fewer N2O producing bacteria (Pseudomonas, Devosia, Luteimonas genus) were observed in the biochar treatment, and thereby decreased the N2O emission. Therefore, 10% BB addition for co-vermicomposting cattle manure and maize straw is an efficient way to increase humification, maturity, and reduce nitrogen loss, and future applications following this strategy is believed to generate better productions.

Problems associated with vermicomposting of dog excrement in practice using Eisenia andrei.

One 25-kg dog produces about 500 g of excrement per day. Excrement is a potentially hazardous material, as it may contain pathogenic microorganisms. Our samples were tested for the presence of thermotolerant coliform bacteria, Enterococcus spp., Escherichia coli and Salmonella spp., which are indicators of faecal contamination, as well as for the presence of helminths and their eggs. During the experiment, it was observed whether these microorganisms could be eliminated by vermicomposting. There were two variants of vermicomposting piles: one test pile (with continuous feeding) and one control pile (with a single feeding). The vermicomposting process was run in outdoor conditions in park for 51 weeks using Eisenia andrei earthworms. The vermicomposting of dog excrement with waste from park maintenance (1:2) can produce a good quality fertiliser. During the process of vermicomposting, there was a gradual decrease in the content of pathogenic bacteria. At the end of the vermicomposting process, there were no eggs or adult helminths. The vermicompost was very rich in microorganisms and enzymatic activity. The pH value was slightly alkaline, and the C:N ratio corresponded to value of mature vermicompost.

Feasibility of housefly larvae-mediated vermicomposting for recycling food waste added digestate as additive.

The development of methods for the efficient treatment and application of food waste digestate is an important research goal. Vermicomposting via housefly larvae is an efficient way to reduce food waste and achieve its valorization, however, studies on the application and performance of digestate in vermicomposting are rarely. The present study aimed to investigate the feasibility of the co-treatment of food waste and digestate as an additive via larvae. Restaurant food waste (RFW) and household food waste (HFW) were selected to assess the effects of waste type on vermicomposting performance and larval quality. Waste reduction rates of 50.9%-57.8% were observed in the vermicomposting of food waste mixed with digestate at a ratio of 25%, which were slightly lower than those for treatments without the addition of digestate (62.8%-65.9%). The addition of digestate increased the germination index, with a maximum value of 82% in the RFW treatments with 25% digestate, and decreased the respiration activity, with a minimum value of 30 mg-O2/g-TS. The larval productivity of 13.9% in the RFW treatment system with a digestate rate of 25% was lower that without digestate (19.5%). Materials balance shows that larval biomass and metabolic equivalent had decreasing trends as the amount of digestate increased and HFW vermicomposting exhibited lower bioconversion efficiency than that of RFW treatment system regardless of the addition of digestate. These results suggest that mixing digestate at a low ratio (25%) during vermicomposting of food waste especially RFW could lead to considerable larval biomass and generate relatively stable residues.

Juxtaposing the quality of compost and vermicompost produced from organic wastes amended with cow dung.

Composting is a propitious technology to change bio-degradable solid waste into organic fertilizers. Considering this, five types of organic waste viz., leaf litter (Tectona grandis), water hyacinth (Eichhornia crassipes), cauliflower waste (Brassica oleracea var. botrytis), coir pith, and mushroom spent waste were composted with and without the use of earthworm (Eisenia fetida). The reaction (pH) and electrical conductivity of compost and vermicompost ranged from 6.98 to 7.45 and 6.97 to 7.36, 0.11 to 0.21 dSm-1, and 0.11 to 0.25 dSm-1, respectively. The chemical oxygen demand both the compost and vermicompost ranged from 687 to 1170 mg l-1 and 633-980 mg l-1 respectively. Cation exchange capacity (CEC) ranged from, 75 to 121 (c mol (p+) kg-1, and 80 to 127 (c mol (p+) kg-1, respectively. The C:N of compost and vermicompost varied from 16:1 to 33:1 and 12:1 to 19:1, respectively. The organic carbon content was decreased (18.3-38.7%), while secondary and micronutrient contents increased over the initial concentration. The NH4+ and NO3- content of compost and vermicompost ranged from 270 to 510 mg kg-1 and 230-430 mg kg-1, 560 to 105 mg kg-1, and 690-1100 mg kg-1, respectively. The nitrification index (NH4+/NO3-) ranged from 0.3 to 0.9 in composts and 0.3 to 0.6 in vermicomposts. The dehydrogenase and urease activity varied from 685 to 1696 µg g-1 hr-1 and 938-2549 µg TPF g-1 day-1 respectively. The bacteria, fungi and actinomycetes population were 2-3, 0.3-0.7 and 3-8 times more in vermicompost over the corresponding compost. This study confirmed that compared to compost, vermicompost showed better nutrients and microbial properties.

Vermiremediation of allopathic pharmaceutical industry sludge amended with cattle dung employing Eisenia fetida.

The present study aims to vermiremediate allopathic pharmaceutical industry sludge (AS) amended with cattle dung (CD), in different feed mixtures (AS:CD) i.e (AS0) 0:100 [Positive control], (AS25) 25:75, (AS50) 50:50, (AS75) 75:25 and (AS100) 100:0 [Negative Control] for 180 days using earthworm Eisenia fetida. The earthworms could thrive and grow well up to the AS75 feed mixture. In the final vermicompost, there were significant decreases in electrical conductivity (29.18-18.70%), total organic carbon (47.48-22.39%), total organic matter (47.47-22.36%), and C: N ratio (78.15-54.59%). While, significant increases in pH (9.06-16.47%), total Kjeldahl nitrogen (69.57-139.58%), total available phosphorus (30.30-81.56%), total potassium (8.92-22.22%), and total sodium (50.56-62.12%). The heavy metals like Cr (50-18.60%), Cd (100-75%), Pb (57.14-40%), and Ni (100-50%) were decreased, whereas Zn (8.37-53.77%), Fe (199.03-254.27%), and Cu (12.90-100%) increased significantly. The toxicity of the final vermicompost was shown to be lower in the Genotoxicity analysis, with values ranging between (76-42.33%). The germination index (GI) of Mung bean (Vigna radiata) showed a value ranging between 155.02 and 175.90%. Scanning electron microscopy (SEM) analysis showed irregularities with high porosity of texture in the final vermicompost than in initial mixtures. Fourier Transform-Infrared Spectroscopy (FT-IR) spectra of final vermicompost had low peak intensities than the initial samples. The AS50 feed mixture was the most favorable for the growth and fecundity of Eisenia fetida, emphasizing the role of cattle dung in the vermicomposting process. Thus, it can be inferred that a cost-effective and eco-friendly method (vermicomposting) with the proper amendment of cattle dung and employing Eisenia fetida could transform allopathic sludge into a nutrient-rich, detoxified, stable, and mature vermicompost for agricultural purposes and further could serve as a stepping stone in the allopathic pharmaceutical industry sludge management strategies in the future.

Role of different earthworm species in nullifying the toxicity of Ipomea carnea and enhancing its utility as a phytoremediator.

The dreaded weed ipomea (Ipomea carnea), has shown promise as a versatile phytoremediator. But I. carnea plants exude several alkaloids and phenols which are harmful to plants as well as animals. Due to this, the weed imparts as much or more toxicity to the soil as it remediates. These authors have earlier found that upon being vermicomposted by Eisenia fetida ipomea loses its toxicity and becomes a benign organic fertilizer with pest repellant attributes. These findings open up the possibility of using earthworms in those segments of land which are sought to be phytoremediated by ipomea so that the earthworms can keep converting the dead ipomea plants and the debris of live plants to fertilizer. The present work has aimed to determine whether the extent and nature of earthworm impact differs from species to species or is similar across different species. It has revealed that the action of each of the four different earthworm species deployed by the authors caused the C:N ratio of ipomea to change drastically ̶ from 28.20 to 15.95 ± 0.75, bringing the vermicomposts to the category of fertilizers fit for horticulture. The Fourier transform infrared (FTIR) spectra revealed that all the species caused a breakdown of the alkaloids and the phenolic compounds present in ipomea, resulting in the weed's detoxification. The earthworms also effected partial degradation of the lignocellulosic content of ipomea to simpler and more soil-friendly constituents like humic acids. Thermogravimetry, differential scanning calorimetry and scanning electron microscopy corroborated these findings. The influence exerted by the four species of earthworms was similarly beneficial in nature and extent.

First-ever study which establishes the general applicability of earthworms in nullifying the toxic impacts caused by ipomea during its use in phytoremediation, thereby greatly enhancing ipomea's value as a phytoremediator. The studies also provide an avenue for the utilization of the otherwise worthless ipomea harvested after phytoremediation or from natural stands.

Biorecovery of olive mill wastewater sludge from evaporation ponds.

Olive mill wastewater (OMW) resulting from the olive oil extraction process is usually disposed of in evaporation ponds where it concentrates generating a sludge that pollutes the ponds nearby area. In this study, four bio-treatments were applied for the in-situ bioremediation and valorization of OMW sludge: Landfarming, phytoremediation, composting and vermicomposting. In all cases, the OMW sludge was added with organic residues (mushroom compost, rabbit manure, and chicken manure). The bio-treatments were carried out in duplicate, inoculated and non-inoculated, to determine the effect of a specialized fungal consortium (Aspergillus ochraceus H2 and Scedosporium apiospermum H16) on the efficacy of the bio-treatments. The evaluation of chemical parameters, toxicity, and functional microbial biodiversity revealed that the four techniques depleted the toxicity and favored the stimulation of functional microbiota. Landfarming and phytoremediation allowed the decontamination and improvement of soils. Composting and vermicomposting also offered high-quality products of agronomic interest. Inoculation improved the bioremediation effectiveness. Biological treatments are effective for the safe recovery of contaminated OMW sludge into high-quality services and products.

Accelerated degradation of cellulose in silkworm excrement by the interaction of housefly larvae and cellulose-degrading bacteria.

The environmental pollution caused by silkworm (Bombyx mori) excrement is prominent, and rich in refractory cellulose is the bottleneck restricting the efficient recycling of silkworm excrement. This study was performed to investigate the effects of housefly larvae vermicomposting on the biodegradation of cellulose in silkworm excrement. After six days, a 58.90% reduction of cellulose content in treatment groups was observed, which was significantly higher than 11.5% of the control groups without housefly larvae. Three cellulose-degrading bacterial strains were isolated from silkworm excrement, which were identified as Bacillus licheniformis, Bacillus amyloliquefaciens, and Bacillus subtilis based on 16S rRNA gene sequence analysis. These three bacterial stains had a high cellulose degradation index (HC value ranged to between 1.86 and 5.97 and FPase ranged from 5.07 U/mL to 7.31 U/mL). It was found that housefly larvae increased the abundance of cellulose-degrading bacterial genus (Bacillus and Pseudomonas) by regulating the external environmental conditions (temperature and pH). Carbohydrate metabolism was the bacterial communities' primary function during vermicomposting based on the PICRUSt. The results of Tax4Fun indicated that the abundance of endo-ß-1,4-glucanase and exo-ß-1,4-glucanase increased rapidly and maintained at a higher level in silkworm excrement due to the addition of housefly larvae, which contributed to the accelerated degradation of cellulose in silkworm excrement. The finding of this investigation showed that housefly larvae can significantly accelerate the degradation of cellulose in silkworm excrement by increasing the abundance of cellulose-degrading bacterial genera and cellulase.

The synergistic effect between biofertility properties and biological activities in vermicomposting: A comparable study of pig manure.

Vermicomposting is a resource technology for managing animal excreta, whereas the internal relationships of the process are vital for its wide applications. The present study examined how macronutrient and micronutrient concentrations, microbial communities, and enzymatic activity of pig manure (PM) changed during the composting and vermicomposting processes and their internal interactions. The vermicomposting process increased macronutrients more significantly than composting (32.40% of total available nitrogen, 21.70% of total available phosphorous, and 12.70% of total available potassium). The vermicomposting reduced total organic carbon (7.91%), C/N ratio (61.35%), and humification index (56.47%) more than composting due to the quick decomposition of PM. After continual fertility optimization, the total microbial population, with the exception of total fungi, rose significantly to accelerate organics mineralization and improve macronutrients in vermicomposting compared to composting. Moreover, earthworm addition favored the stabilization of the PM containing higher concentrations of micronutrients after being catalyzed by the enhanced catalase activity and reduced sucrase activity after 90 days of vermicomposting. Principal component analysis and chord plots found that the generated vermicomposting products had higher fertility properties and biological activities induced by the synergistic effect of microorganisms and earthworms. These findings highlight vermicomposting is an eco-friendly management technology for processing PM and can be scaled up for agricultural applications.