Findings consistent with equine proximal suspensory desmitis can be reliably detected using computed tomography and differ between affected horses and controls.

The objective of this retrospective, observational, controlled study was to evaluate bone and soft tissue window CT images of the proximoplantar metatarsus III region in twenty horses with pain localized to the proximal suspensory ligament (PSL) and 20 horses with findings nonrelated to tarsal pain. All horses underwent CT and radiographic examination. Images were reviewed by three independent observers who graded the severity and localization of findings. Bone-related categories as well as soft tissue-related categories were evaluated. For the comparison of imaging findings in horses with and without proximal suspensory desmitis (PSD), mixed linear regression was performed. The intraclass correlation coefficient (ICC) was calculated to assess intraobserver agreement, and kappa statistics were employed to evaluate interobserver agreement. CT examination identified significantly more abnormalities in the diseased group. The scores for osseous exostosis (p = .015) and PSL enlargement (p = .004) were notably higher in PSD horses compared to controls. Intraobserver agreement was overall high (ICC .82-1.0), and interobserver agreement was substantial for the detection of mineralization (kappa = .61) and moderate for sclerosis (kappa = .43), exostosis (kappa = .43), and PSL enlargement (kappa = .48/.51). Measurements in the soft tissue window were significantly smaller than those in the bone window. Findings concurrent with PSD including osseous proliferation and sclerosis as well as soft tissue enlargement, mineralization, and avulsion can be reliably detected using CT. Findings from the current study supported the use of CT for evaluating horses with suspected PSD where high-field MRI is not available.

Ultrasonographic comparison of the intermediate patellar ligament in warmbloods versus quarter horses.

Striations are present on ultrasonography of the intermediate (middle) patellar ligament in the transverse plane, which can be confused with tears. Comparison to the contralateral limb is often performed to help differentiate anatomic variation from pathologic change. The purposes of this prospective, observational study were to describe the striation patterns in Warmbloods and Quarter Horses, determine if these patterns are bilaterally symmetrical, and compare striation characteristics between Warmbloods and Quarter Horses. The intermediate patellar ligaments of six Warmblood horses and six Quarter horses, free from clinical signs of hindlimb lameness and in full work, were examined ultrasonographically. Striation pattern, striation number, and the transverse-sectional areas for intermediate patellar ligaments were compared between Warmbloods and Quarter horses. Striation patterns were also compared for the left and right limbs of each horse. A significant difference between breeds was identified at the mid-portion of the intermediate patellar ligament when both left and right ligaments were included (P = 0.02) and when comparing the right intermediate patellar ligament (P = 0.02). There were no other significant breed differences, and a parallel pattern was the most common pattern type. Two-thirds of the horses in this study had bilaterally symmetrical patterns within the distal aspect of their intermediate patellar ligaments, while one-third of the population were asymmetric. These results show that comparison of the contralateral limb during ultrasonography to determine if changes within the distal aspect of the intermediate patellar ligament are normal striations versus tears may lead to misdiagnosis in one-third of horses, regardless of breed. Other signs of pathology on ultrasonography should be used instead.

Relationships between type of hoof lesion and behavioral signs of lameness in Holstein cows housed in Canadian tiestall facilities.

Although foot pain can affect gait, the presence of a hoof lesion may or may not cause the cow to show visible changes in their gait. This can be dependent on the type and severity of the lesion; for example, the presence of a sole ulcer (SU) has been associated with increased gait scores, whereas digital dermatitis (DD) and sole hemorrhage (SH) have not. In tiestall facilities, gait scoring can be difficult to perform. An alternative method, known as stall lameness scoring (SLS), allows observers to assess cattle for lameness while they remain in their stall. Lameness is determined based on behavioral changes in weight bearing and foot positioning, which include: shifting weight, resting a foot, standing on the edge of the stall, and uneven weight bearing when stepping side to side. The aim of this study was to examine relationships between hoof lesions and these behavioral indicators. A total of 557 observations of SLS and corresponding hoof trimming records, collected during routine trimming events on 7 tiestall herds, were obtained. Trimming was performed by 2 trained hoof trimmers with good agreement on lesion identification, based on quizzes taken at the beginning and mid-way through the study. To ensure trimming had no effect on the behavioral indicators observed, SLS was always performed by a trained observer before trimming. Behavioral indicators focused on the hind limbs only; therefore, the analysis was confined to hind limb lesions using logistic regression to detect the presence of hoof lesion based on observations made during SLS. Seventy-five percent of observed cows had no SLS behavioral indicators, whereas, 11, 12, and 1% had 1, 2, and 3 behavioral indicators, respectively. At least one hind limb lesion was noted during trimming in 19% of cows, with the most common lesions being DD (7%), SU (6%), and SH (4%). A cow that was observed resting one foot and bearing weight unevenly when moving side to side had higher odds of having a hind limb hoof lesion than a cow not displaying these behaviors. When looking at specific hoof lesions, a cow observed resting one limb and bearing weight unevenly had higher odds of having a SU compared with those not displaying these behaviors. A cow observed shifting their weight from one foot to another had higher odds of having SH, and a cow observed bearing weight unevenly had higher odds of DD. Behavioral indicators in weight bearing and foot positioning can help identify cows in tiestalls with hind limb hoof lesions. Producers could routinely observe their cattle for these indicators to assist in the identification of cows that may require treatment. This could help reduce the duration of clinical lameness through earlier intervention.

In vitro biomechanical properties of sole tissues: Comparison between healthy and ulcerated bovine claws.

Sole ulcers are reportedly one of the most prevalent diseases associated with lameness in dairy cattle, significantly affecting animal welfare and farm profitability. The degree to which sole soft tissues, healthy or ulcerated, are able to maintain their structure and function when subjected to compressive forces remains unknown. Therefore, the aims of the present study were to assess sole tissue biomechanics in healthy and ulcerated claws and to describe correlated histology. Cylindrical samples were harvested from zones 4 and 6, as described by the international foot map, from hind lateral healthy (n = 12) and ulcerated bovine claws (n = 8; animals n = 12). Tissue biomechanics and morphology were evaluated via compressive tests and hematoxylin-eosin-phloxine-saffron staining, respectively. A 2-sample t-test was used to compare zones' mechanical properties between healthy and ulcerated tissues, and the Cochran-Mantel-Haenszel test was used to measure the effect of claw zone on histology. The fibril modulus (Ef) and permeability (k) respectively increased and decreased in ulcerated claws (Ef = 0.201 ± 0.104 MPa; k = 0.128 ± 0.069 mm2/MPa·s) compared with healthy claws (Ef = 0.105 ± 0.050 MPa; k = 0.452 ± 0.365 mm2/MPa·s) only for zone 6. Histology scores equal to or greater than 3 were associated with macroscopic presence of ulceration. A higher proportion of adipose tissue (30% or more) was associated with zone 6 compared with zone 4, but no difference was seen between healthy and ulcerated claws. Ulcerated claws had a higher prevalence of exostoses compared with healthy ones (33% vs. 8%). Sole soft tissues showed, as hypothesized, a viscoelastic behavior using unconfined compression testing, which, however, may not reflect in vivo loading conditions. Clinical and histological signs of sole ulceration were not associated with decreased strength of the supportive apparatus of the distal phalanx in zone 4 in this study.

Association between hoof lesions and fertility in lactating Jersey cows.

The objectives of this study were to evaluate the association between hoof lesions and fertility in dairy cows. Lactating Jersey cows (n = 1,639) were enrolled at 20 ± 3 d in milk (D20), examined and treated for presence of hoof lesions (HL), and evaluated for body condition score (BCS). Afterward, they were managed according to standard farm procedures, including estrus detection and presynchronization and a 5 d Cosynch-72 protocol for cows that failed to show estrus. Ovaries were scanned at 27 and 41 ± 3 d in milk, and cows with a corpus luteum greater than 20 mm on at least 1 exam were considered cyclic. At 120 ± 3 d in milk (D120), cows were re-examined for HL and BCS. Cows were classified at D20 according to HL status as healthy (n = 1,197) or having HL (n = 429), and according to HL category as healthy (n = 1,197) or having a sole hemorrhage (n = 280), noninfectious HL (sole ulcer, toe ulcer, or white line disease; n = 113), or infectious HL (digital dermatitis and foot rot; n = 36). Cows with HL at D20 had reduced odds of being cyclic (38.3 vs. 51.9%) and a longer interval from calving to first service (58 vs. 51 d) compared with healthy cows. Cows with infectious HL at D20 had reduced odds of pregnancy to first service (16.7 vs. 38.3%) compared with healthy cows. Cows with sole hemorrhage at D20 were more likely to lose pregnancies between d 32 and 64 after the first service postpartum compared with healthy cows (10.5 vs. 5.2%). Cows with sole hemorrhage at D20 had a smaller hazard of pregnancy (67.9 vs. 75.5%) at 150 d in milk and more days open (88 vs. 77d) compared with healthy cows. To assess the relationship between the development of HL and fertility, cows were classified as healthy (no HL at D20 and D120; n = 308), cured (any HL at D20 and no HL at D120; n = 72), new HL (no HL at D20 and any HL at D120; n = 597), and chronic (any HL at D20 and D120; n = 226). Sole hemorrhage accounted for 93% of new HL. The proportions of cows with HL at D20 and D120 were 26.9 and 68.4%, respectively. We found no evidence for a difference in pregnancy hazard at 150 d in milk between cows that remained healthy (n = 308) and cows that developed new HL (n = 597). Hoof lesions at D20, but not new HL, were associated with decreased odds of cyclicity, longer interval from calving to first service postpartum, and reduced pregnancy hazard in Jersey cows. The effect of an HL diagnosis in early lactation and management to reduce chronic HL in dairy cows warrants further investigation.

Short communication: Accuracy of estimation of lameness, injury, and cleanliness prevalence by dairy farmers and veterinarians.

Lameness, injuries, and cleanliness are considered important indicators of dairy cow welfare, milk production, and milk quality. Previous research has identified that farmers globally underestimate the prevalence of these cow-based measurements, but no information on the perceptions of veterinarians is available. Because veterinarians are often perceived as the main providers of health advice on farms, the objective of the present study was to evaluate the relationship between the true prevalence of lameness, injury (hock, knee, neck), and cleanliness (udder, legs, flanks), and the estimated prevalence of these issues by farmers and their herd veterinarians. A cross-sectional study was conducted between February 2016 and July 2017. First, the farm owner and the herd veterinarian were asked to estimate the prevalence of lameness, of neck, knee and hock injuries, and of udder, leg, and flank cleanliness on the farm. The research team then visited the farm and scored all lactating cows in the herd for each measurement. Linear regression models were used to assess the relationship between the prevalence estimated by the veterinarians and the farmers, of each cow-based measurement, and the true prevalence on the farm. The 93 herds enrolled had a median of 55 milking cows and were housed in tiestall (90%) and freestall (10%) barns. Ten herd veterinarians participated and were involved with 2 to 22 enrolled farms each. A wide variation was detected in the true prevalence of the different cow-based measurements among herds (lameness: range = 19-72%, median = 36%; neck injuries: range = 0-65%, median = 14%; knee injuries: range = 0-44%, median = 12%; hock injuries: range = 0-57%, median = 25%; dirty udder: range = 0-55%, median 13%; dirty legs: range = 0-91%, median = 18%; and dirty flanks: range = 0-82%, median = 20%). For both veterinarians and farmers, the perception of each cow-based measurement prevalence increased incrementally as the herd's true prevalence increased. Overall, farmers and veterinarians underestimated cow-based measurements. Farmers and veterinarians more accurately estimated lameness prevalence in herds with higher prevalence than in herds with low prevalence, suggesting a better awareness of the issue on farms with lameness problems. Injuries were less accurately estimated in herds with higher injury prevalence compared with herds with lower prevalence, suggesting an opportunity for better knowledge transfer in this area.

Association between lameness risk assessment and lameness and foot lesion prevalence on dairy farms in Alberta, Canada.

Lameness is a detrimental health and welfare concern of dairy cattle with high prevalence in North American herds. As a practical and farm-specific approach toward its mitigation in Alberta, Canada, a score-based lameness risk assessment questionnaire (RAQ) was developed for veterinarians to conduct with their clients; however, its accuracy in identifying risk for lameness and hoof lesions has not been evaluated. Our objectives were to (1) provide an update on prevalence of lameness and hoof lesions in Alberta freestall herds, (2) validate the RAQ by determining the association between RAQ scores and the prevalence of herd lameness and herd lesions, and (3) determine the association between presence of common hoof lesions and lameness. For the first 2 objectives, 65 Alberta dairy farms were visited between March and October 2018 to collect a completed RAQ, hoof trimming records from their hoof trimmer, and video footage of the entire lactating herd. For the third objective, 10 participant herds were visited twice between November 2013 and June 2014 to take video footage of the entire lactating herd and collect hoof trimming records from their hoof trimmer within 48 h after video footage collection. Median herd-level lameness prevalence was 20% (range = 2-56%), infectious lesion prevalence was 10% (range = 0-49%), and noninfectious lesion prevalence was 15% (range = 2-39%). Of cows that were lame, 93% had a lesion, whereas 21% of cows with a lesion were lame. Cows with an infectious and noninfectious lesion were, respectively, 1.5 and 3.1 times more likely to be lame than cows with no lesion. Total RAQ score and herd lameness prevalence were correlated (r = 0.26) and noninfectious RAQ score and noninfectious lesion prevalence were correlated (r = 0.45), whereas infectious RAQ score and infectious lesion prevalence were not. Cow-level mixed logistic regression models indicated no associations between any of the RAQ scores and presence of lameness or a hoof lesion. Lameness prevalence in Alberta remains high, indicative of a low adoption rate of mitigation strategies by producers. Improvement of the RAQ could allow for better reflection of lameness and lesions on farm and can be achieved through further risk factor analysis within the RAQ, which may result in removal or addition of questions as well as the adjustment of scores based on risk factors' strength of association with lameness and lesions.

Interobserver agreement of digital dermatitis M-scores for photographs of the hind feet of standing dairy cattle.

Digital dermatitis (DD) is the leading infectious cause of lameness in dairy cattle, and it affects their welfare and productivity worldwide. At the herd level, DD is often assessed while cows are standing in a milking parlor, and lesions are most commonly evaluated using the M-score. The objective of this study was to examine the interobserver agreement for M-scores of the feet of standing cattle, based on digital color photographs of dairy cattle hind feet. A total of 88 photographs and written descriptors of the M-score were sent to 11 scorers working at 10 different institutions in 5 countries. The scorers received no formal training immediately before scoring the photographs; however, all regularly used the M-score to score DD. The answers for 36 photographs were excluded from the analysis because the photograph either had more than 1 M-stage as mode or not all scorers assigned an M-score to it. The M-scores of the 11 scorers from 52 photographs were available for analysis. Interobserver agreement was tested using Gwet's agreement coefficient (AC1) and the mode was assumed correct. Overall, moderate agreement emerged for the M-score (AC1 = 0.48). For the individual M-stages, almost perfect agreement existed for M0 (AC1 = 0.99), M1 (AC1 = 0.92), and M3 (AC1 = 0.82), and substantial agreement for M2 (AC1 = 0.61), M4 (AC1 = 0.65), and M4.1 (AC1 = 0.71). This outcome indicates the degree of individual variation in M-scoring in this context by unstandardized, experienced European observers, particularly for the M2, M4, and M4.1 stages. Standardized training is likely to improve the consistency of M-scoring and thus the generalizability of future DD research results on this important endemic disease.

Fluorine-18-fluoro-2-deoxy-d-glucose PET-CT aids in detection of soft-tissue injuries for dogs with thoracic or pelvic limb lameness.

Fluorine-18-fluoro-2-deoxy-d-glucose positron emission tomography (FDG PET) provides physiologic images of tissues based on their glucose metabolism. The combination of FDG PET and CT (FDG PET-CT) has been utilized in human musculoskeletal imaging to localize soft tissue lesions, however, this modality has not been thoroughly investigated for the diagnosis of canine lameness. This prospective, descriptive study evaluated FDG PET-CT findings in 25 client-owned dogs with inconclusive origin of thoracic or pelvic limb lameness (thoracic limb n = 15/25, 60%; pelvic limb n = 6/25, 24%; and combination of both limbs n = 4/25, 16%). We hypothesized that FDG PET-CT would aid the detection of soft tissue lesions not visible with other imaging modalities. Combined FDG PET-CT detected soft tissue lesions in 40% (n = 10/25) and osteoarthritis in 64% (n = 16/25) of the patients. FDG PET detected more soft tissue lesions than contrast-enhanced CT (n = 15/15, 100% and n = 12/15, 80%, respectively), while CT identified more osteoarthritis lesions than FDG PET (n = 26/26, 100% and n = 18/26, 69%, respectively). The three imaging-diagnoses based on the FDG PET component included the following: flexor carpi ulnaris muscle tear, psoas major myopathy, and tarsal desmopathy. No diagnosis for the lameness was obtained in three dogs. Findings supported FDG PET-CT as a useful adjunct imaging modality for detection of certain soft tissue injuries of the musculoskeletal system. Combined FDG PET-CT should be considered for cases where the cause of lameness is thought to be of soft tissue origin and cannot be diagnosed by conventional means.

Caprine herpesvirus 2-associated malignant catarrhal fever of captive sika deer (Cervus nippon) in an intensive management system.

BACKGROUND: Caprine herpesvirus 2 (CpHV-2) infection usually induces chronic malignant catarrhal fever (MCF) in sika deer (Cervus nippon), with the primary signs of weight loss, dermatitis and alopecia. CASE PRESENTATION: Here, we report a case of CpHV-2-associated acute MCF in a sika deer herd raised in an intensive management system distant to the reservoir goats. Affected deer developed clinical signs of high fever (41 °C) followed by nasal discharge and lameness. Severe lesions of hemorrhage, necrosis and infiltration of lymphoid cells could readily be observed in the lung, kidney, heart valves and subcutaneous tissue surrounding a tendon. Etiologically, identical CpHV-2 specific DNA sequences were detected in peripheral blood lymphocyte (PBL) from the affected deer and reservoir goats. CONCLUSION: In summary, domestic goats were the reservoir of the CpHV-2, which is the causative agent of the outbreak of MCF in the three hinds. The disease was probably transmitted via aerosol infection. In addition, necrosis and inflammation in subcutaneous tissue surrounding a tendon was the reason for lameness. Therefore, MCF should be put into a differential diagnostic list when similar disease occurs in sika deer herds.

Prevalence of lameness and leg lesions of lactating dairy cows housed in southern Brazil: Effects of housing systems.

Within the last few decades, the North American and European dairy industries have been collecting information about lameness and leg injury prevalence on dairy farms and have tried to develop solutions to mitigate these ailments. Few published articles report the prevalence of lameness and leg lesions in areas outside of those 2 regions, or how alternative housing systems, such as compost-bedded packs, affect the prevalence of these maladies. The objectives of this study were to compare the prevalence of lameness and leg lesions on confined dairies that used freestall, compost-bedded packs, or a combination of these 2 systems in Brazil. Data were collected in the autumn and winter of 2016 from 50 dairy farms located in Paraná state, including 12 compost-bedded pack dairies (CB), 23 freestall dairies (FS), and 15 freestall dairies that used compost-bedded packs for vulnerable cows (FS+C). A visit to the farm consisted of a management questionnaire, an inspection of the housing areas as well as the milking parlor, and an evaluation of all lactating cows as they exited the parlor for lameness (score 1-5), hygiene (score 0-2), body condition score (score 1-5), and hock and knee lesions (score 0-1). Median 1-way chi-squared test was used to compare production systems. We found no difference between farm types in management practices related to hoof health management or average daily milk production per cow [31 (29-33.9) kg/d; median (quartile 1-3)], percentage of Holstein cattle in the herd [100% (90-100%)], conception rate [35.8% (30.2-38%)], or pregnancy rate [15% (13.7-18%)]. The CB farms were smaller [85 (49.5-146.5) milking cows] than both the FS [270 (178-327.5) milking cows] and FS+C farms [360 (150-541.5) milking cows). The overall prevalence of severe lameness (score 4 and 5) across all farms was 21.2% (15.2-28.5%) but was lower on the CB farms [14.2% (8.45-15.5%)] in comparison to the FS [22.2% (16.8-26.7%)] and the FS+C farms [22.2% (17.4-32.8%)]. Less than 1% of all cows scored on CB farms were observed with swollen or wounded knees (or both), which was lower than either the FS or FS+C farms [7.4% (3.6-11.9%) and 6.4% (2.6-11.8%) of all cows scored, respectively]. The same pattern was found for hock lesions, where the farm-level prevalence within the 3 different housing types was 0.5% (0-0.9%), 9.9% (0.8-15.3%), and 5.7% (2.6-10.9%) for CB, FS, and FS+C farms, respectively. No differences between farm systems were observed for hygiene or body condition score. On average, 2.7% (0.8-10.9%) of lactating cows had a soiled side, 15.4% (2.1-37.4%) had dirty legs and 1.7% (0-9.3%) had dirty udders. The average herd-level body condition score across farms was 2.9 (2.9-3), with 0.86% of the all cows scored having a body condition score <2.5. These results indicate that lameness prevalence on confined dairies in Brazil is high and highlight the need for remedial changes in environmental design and management practices. We found that CB farms in this region had reduced lameness and lesions in relation to FS or FS+C dairies.

Additional palmaroproximal-palmarodistal oblique radiographic projections improve accuracy of detection and characterization of equine flexor cortical lysis.

Radiographic flexor cortical lysis indicates advanced degenerative change and its earlier recognition may improve case outcome. Aims of this prospective, diagnostic accuracy study were to determine effects of radiographic beam angle and observer on accuracy of lesion detection. The sample included 36 limbs from 31 horses. Palmaroproximal-palmarodistal oblique (skyline) radiographs were acquired at standard (n = 38) and more shallow (alternate) angles (n = 58). Images were independently reviewed by four experience levels of five observers each (n = 20) for the presence and severity of flexor cortical lysis. Observers also reported their confidence in these answers. Responses were compared based on seeing a standard skyline or multiple projections. The definitive presence (or absence) and severity of lysis was based upon radiologist consensus agreement. When assessed by observer, the identification of lysis and the assessment of its severity was most similar to that of radiologists when observers of all levels of experience were able to view multiple skyline projections (P = 0.399, P = 0.174). Using multiple views to detect lysis resulted in improved sensitivity (85.3% vs. 97.2%, P < 0.001), decreased specificity (82.8% vs. 74.5%, P = 0.03), and improved interobserver agreement (86.0% vs. 90.2%, P = 0.21). On average, observers of all levels of experience became more confident viewing multiple projections (P < 0.001). Skyline radiographs using a flatter angle of incidence improve radiographic detection and characterization of flexor cortical lysis severity, may allow the beam to be tangential to the more distal portion of the navicular bone, and are recommended when flexor cortical lysis is suspected.

High field magnetic resonance imaging contributes to diagnosis of equine distal tarsus and proximal metatarsus lesions: 103 horses.

Injury of the distal tarsus and proximal metatarsus commonly causes lameness. Magnetic resonance imaging (MRI) allows concurrent assessment of both the distal tarsal joints and suspensory ligament origin, and aids identification of lesions that may otherwise go undetected by other modalities. In this retrospective observational study, the medical records of a veterinary imaging center were searched for MRI exams of the distal tarsus and proximal metatarsus for the years 2012 through 2014. Studies for 125 limbs of 103 horses were identified and retrospectively evaluated by two board-certified veterinary radiologists. Soft tissue and osseous changes were characterized and graded by degree of severity. The patients' signalment, lameness severity, and results of diagnostic analgesia were recorded. Osteoarthritic changes of the distal intertarsal and tarsometatarsal joints were the most common findings. Other findings included bone marrow lesions, degenerative changes of the small cuboidal bones, subchondral cystic lesions, and intertarsal desmopathy. Suspensory ligament desmopathy was found in 53% of limbs. Fourty-seven percent of limbs that responded to analgesia of the proximal suspensory ligament had more severe lesions in the distal tarsus. Bone marrow lesions of the third tarsal bone were the only MRI finding that correlated with grade of lameness in patients for which lameness grade was reported. The grade of lameness has a poor correlation with the severity of lesions found on MRI. The findings support the use of MRI for simultaneous evaluation of the proximal metatarsus and distal tarsus, particularly given the difficulty of lesion localization with diagnostic analgesia.

B-mode and power Doppler ultrasonography of the equine suspensory ligament branches: A descriptive study on 13 horses.

Ultrasonography is routinely used to achieve the diagnosis of equine suspensory ligament desmopathy. In human medicine, power Doppler ultrasonography has also been found to be useful for the diagnosis of tendon/ligament injuries. The aim of this prospective, pilot study was to assess the presence or absence of power Doppler signal in suspensory ligament branches and compare B-mode findings with power Doppler findings in suspensory ligament branches of lame and non-lame limbs. Thirteen horses were used (eight lame horses, with lameness related to pain in the suspensory ligament branches, and five non-lame horses). Ten lame limbs and 24 sound limbs were assessed by B-mode and power Doppler ultrasonography. The severity of power Doppler signal was scored by two independent readers. The B-mode ultrasonographic examination revealed abnormalities in branches of lame limbs and in branches of sound limbs. Suspensory ligament branches that were considered normal in B-mode showed no power Doppler signal. However, power Doppler signal was detected in suspensory ligament branches that were abnormal in B-mode, both in lame and sound limbs. Power Doppler scores were subjectively higher in suspensory ligament branches of lame limbs and in branches with more severe B-mode changes. Findings supported the use of power Doppler as an adjunctive diagnostic test for lame horses with suspected suspensory desmopathy.

Evaluation of the diagnostic accuracy of skeletal scintigraphy in lame and poorly performing sports horses.

Skeletal scintigraphy is an established imaging modality, however, its validity as a diagnostic test in lame or poorly performing sports horses has not been determined. The objective of this retrospective cross-sectional analytical study was to determine the accuracy of skeletal scintigraphy as an indiscriminate screening test in sports horses. All sports horses that underwent scintigraphic examination between March 2008 and December 2014 for which a definitive diagnosis was reached were included. Examinations were evaluated blindly. Characteristics of increased radiopharmaceutical uptake were recorded and subjectively categorized as possibly relevant or non-relevant. Kappa statistics were used to assess agreement between test results and final diagnosis. There were 1222 positive clinical diagnoses in 480 horses, of which 687 regions (56.4%) had no associated increased radiopharmaceutical uptake and 99 regions (8.1%) had non-relevant increased radiopharmaceutical uptake. The sensitivity of increased radiopharmaceutical uptake for identification of final diagnosis was low (43.8%) and specificity was high (94.0%). The agreement was fair (κ = 0.36). Relevance categorization improved the test; the sensitivity for final diagnosis was low (35.7%), specificity was high (98.9%), and the agreement was moderate (κ = 0.45). The agreements of increased radiopharmaceutical uptake and relevance categorization with final diagnosis were only substantial for feet (κ = 0.67 and κ = 0.66, respectively) and proximal aspect of the forelimb (elbow, shoulder, and scapula combined) (κ = 0.69 and 0.78, respectively). Scintigraphic examination is unlikely to lead to a full and correct diagnosis of the cause(s) of lameness or poor performance in sports horses when used as an isolated or indiscriminate screening tool.

Evaluation of the diagnostic accuracy of skeletal scintigraphy for the causes of front foot pain determined by magnetic resonance imaging.

There is good correlation between increased radiopharmaceutical uptake anywhere in the feet of sports or leisure horses and the presence of foot pain. However, low sensitivity of scintigraphy for identifying lesions contributing to foot pain as determined by magnetic resonance imaging (MRI) has been reported. The aim of this retrospective, cross-sectional, analytical study was to assess the accuracy of scintigraphy for identifying the cause(s) of foot pain, using MRI as the reference standard. Sports and leisure horses that underwent both skeletal scintigraphy and MRI of the front feet between March 2008 and December 2014 and for which a definitive diagnosis of the cause(s) of front foot pain was reached were included (121 feet of 70 horses). Imaging studies were evaluated retrospectively, blindly. Agreements between scintigraphic localization of increased radiopharmaceutical uptake and subjective categorization of lesions with MRI diagnosis were assessed using Kappa statistics (κ). Measures of diagnostic accuracy were calculated. Sensitivity of increased radiopharmaceutical uptake varied among regions (31.8-76.2%) but specificity was at least high for all (84.6-100%). Agreements between both scintigraphy measures and MRI diagnosis were substantial for the distal phalanx (palmar processes and body) (κ = 0.77 and κ = 0.74, respectively), and ungular cartilages and chondral ligaments (κ = 0.62, for both). When all regions were combined, agreements of increased radiopharmaceutical uptake (κ = 0.49) and relevance categorization (κ = 0.45) with MRI diagnosis were moderate. In conclusion, skeletal scintigraphy does not meet the minimum reliability level for a diagnostic test, but is reliable for identification of osseous trauma to the distal phalanx and ungular cartilages.

[Correlation of a lameness scoring system and claw lesion]. / Korrelation eines Lahmheit-Scoring- Systems und Klauenläsionen.

INTRODUCTION: The aim of this study was to investigate the relationship between lameness scoring and clinical findings. The lameness scoring and a gait assessment took place during drying off, the first week after calving and two months after calving in 29 lame and 27 free of lameness cows. In between the farmer scored the lamness. Most of the lameness occurred in the hind limbs and were most frequently caused by dermatitis digitalis, Mortellaro, Limax, and Rusterholz sole ulcer. The mean duration of lameness after treatment was 26 days (median 16 days) and was significantly longer in case of horn lesions than in skin lesions (including interdigital dermatitis). Lameness- free control animals were often affected by heel horn erosion and digital dermatitis. It was shown that the sensitivity of lameness (lameness is caused by a certain aetiology) for a certain cause of lameness (dermatitis interdigitalis, doble sole, purulent hollow wall, Limax, digital dermatitis, Rusterholz sole ulcer and sole contusion) increases along with the threshold (degree of lameness) but that the specificity (no lameness is not related to a certain aetiology) remained constant at the same time. The highest sensitivity was achieved in injuries, followed by interdigital hyperplasia. The receiver operating curve (ROC) showed that lameness was the best selecting symptom in white line lesions followed by claw injury. The positive predictive values varied between 38.8% in white line lesions and 0% in Rusterholz's sole ulcer. The negative predictive values varied between 99.1% in interdigital dermatitis and 82.3% in interdigital dermatitis. The ROC analysis shows which lameness is more related to a certain claw disease. The results demonstrate, that a lameness score cannot be linked to a certain claw disease.

INTRODUCTION: Le but de la présente étude était de voir dans quelle mesure il existe une relation entre un système de scoring des boiteries et les constatations cliniques. On a utilisé un scoring de boiterie simple et une estimation de la marche chez 29 animaux boiteux et 27 animaux sains au moment du tarissement, dans la première semaine après le vêlage ainsi que deux mois après celui-ci. Entre ces examens, les boiteries ont été enregistrées par les agriculteurs. La plupart des boiteries affectaient les postérieurs et étaient le plus souvent causées pas une dermatite digitée, une limace ou un ulcère de Rusterholz. La durée moyenne de la boiterie après traitement était de 26 jours (médiane 16 jours) et était nettement plus importante lors de lésions de la corne que lors de lésions de la peau. Les animaux non-boiteux étaient souvent atteints de pourriture de la corne en talons et de dermatite digitée. On a constaté que la sensitivité de la boiterie (la boiterie parle pour une cause donnée) augmentait avec l'augmentation de la valeur limite (intensité de la boiterie) pour une cause de boiterie donnée (dermatite interdigitée, double sole, fourmilière purulente, limace, dermatite digitée, ulcère de Rusterholz, contusion de sole) et que, simultanément, la spécificité (l'absence de boiterie parle contre une certaine cause) restait constante. La sensitivité la plus élevée était observée en cas de blessures, suivies par les limaces. La surface sous la courbe ROC (receiver operating curve) montrait que la discrimination était la meilleure en cas de fourmilière purulente, suivie par les blessures d'onglon. Les valeurs prédictives positives variaient entre 38.8% pour la fourmilière purulente et 0% pour l'ulcère de Rusterholz. Les valeurs prédictives négatives variaient entre 99.1% pour la dermatite interdigitée et 82.3% pour la dermatite digitée. L'analyse ROC montre quelles boiteries sont associées le plus souvent avec certaines affections des onglons. Les résultats montrent qu'on ne peut pas, sur la base de la boiterie, déduire l'affection des onglons.

Pathological and microbiological investigations into cases of bacterial chondronecrosis and osteomyelitis in broiler poultry.

Bacterial chondronecrosis and osteomyelitis (BCO) is increasingly recognized as a major cause of lameness in commercial broilers chickens worldwide, but the pathogenesis of the condition is incompletely understood. This was a longitudinal study of 20 commercial broiler farms in Victoria, Australia, to investigate the aetiology and pathology of BCO. Thorough postmortem examination was performed on culled and dead birds (n = 325) from 20 different flocks at either 1 week, 4 weeks or 5 weeks of age and samples were analysed by conventional bacteriology, molecular identification of infectious organisms detected, serology and histopathology. BCO occurs throughout the life of broiler flocks at a very high rate, with lesions detected in 28% (95% CI 23-34%) of the mortalities and culls. The condition occurs with similar prevalence in both the femur and tibiotarsus. BCO is an infectious process that appears to result from bacteraemia and haematological spread of bacterial pathogens, especially Escherichia coli, to the bones, with 65.3% bacterial isolates from histologically confirmed BCO identified as E. coli, 11.5% as Staphylococcus and the remainder composed of mixed infections or a range of other minor isolates. We observed that almost all E. coli isolated from cases of BCO are avian pathogenic E. coli, suggesting that preventative measures should be directed at this organism.

Growth Plate Lesions of Fattening Bulls.

Lameness related to growth plate lesions is an important problem in the beef industry. This article describes the macroscopic and microscopic lesions in the distal metatarsal physis of bulls from an association of farmers in northeastern Italy. The metatarsal bones of 62 bulls (12 with severe lameness and 50 without lameness), average age 16.44 ± 1.72 months, were examined at the abattoir. The animals came from the same geographic area and shared intensive husbandry practices and a diet based on maize starch. A total of 124 metatarsal bones were sectioned, and the distal metaphyseal growth plate was grossly examined. Twenty-three cases, including 12 lame and 9 nonlame animals with visible lesions on macroscopic examination, and 2 controls (a total of 46 physes) were examined microscopically. Eight of 12 bulls with severe lameness had a chronic purulent physitis in at least 1 limb. Segmental thickening of the hypertrophic zone, consistent with osteochondrosis (OC), was present contralaterally ( n = 3 cases) and bilaterally ( n = 3 cases) in 6 of these animals. In the group of nonlame bulls, 19 of 50 (38%) had similar segmental thickening of the physis consistent with OC. In the remaining bulls, minor findings included partial closure of the physis and a variable degree of metaphyseal hyperemia. A high incidence of OC was found in both lame and nonlame fattening bulls. It is likely that lame animals were clinically more severe due to secondary hematogenous implantation of bacteria, resulting in a purulent physitis and severe lameness that required emergency slaughter in some cases.

Semitendinosus myopathy and treatment with adipose-derived stem cells in working German shepherd police dogs.

Semitendinosus myopathy has been treated with numerous surgical and non-surgical therapies resulting in recurrence of lameness within 2 to 9 months. Eleven cases of semitendinosus myopathy diagnosed in 8 working police dogs that were treated with adipose-derived mesenchymal stem cells were retrospectively evaluated. At short-term follow-up < 6 mo, ultrasound and gait evaluations revealed a mean reduction in the overall intramuscular lesion size of 54.82% (SD +/- 18.02; range: 30.5% to 82.7%) and reduction in the Visual Assessment Score (VAS) of 1 to 3 points. At long-term follow-up > 1 y, in 8 cases the dogs had a normal gait and in 3 cases the dogs had an improved gait compared with initial examination, and all 8 dogs returned to active police work. Fisher's exact test resulted in P = 0.000008 when comparing published historical reports and these 11 cases for resolution of lameness and return to active duty.

Myopathie du muscle semi-tendineux et traitement à l'aide de cellules souches adipeuses chez des chiens policiers Bergers allemands. La myopathie du muscle semi-tendineux a été traitée à l'aide de nombreuses thérapies chirurgicales et non chirurgicales qui ont produit une récurrence de la boiterie dans un délai de 2 à 9 mois. Onze cas de myopathie du muscle semi-tendineux diagnostiqués chez 8 chiens policiers qui avaient été traités à l'aide de cellules souches mésenchymateuses adipeuses ont été évalués rétrospectivement. Au suivi à court terme de < 6 mois, les évaluations de l'échographie et de la démarche ont révélé une réduction moyenne de la taille de la lésion intramusculaire totale de 54,82 % (SD +/− 18,02; écart : de 30,5 % à 82,7 %) et une réduction de la note d'évaluation visuelle (NÉV) de 1 à 3 points. Au suivi à long terme de > 1 an, 8 cas avaient une démarche normale et 3 avaient une démarche améliorée comparativement à l'examen initial et les 8 chiens sont retournés au travail policier actif. La méthode exacte de Fisher s'est traduite par un résultat de P = 0,000008 lors de la comparaison avec des rapports historiques publiés et ces 11 cas de résolution de boiterie et de retour au service actif.(Traduit par Isabelle Vallières).