Effect of varying hypoxia reoxygenation times on autophagy of cardiomyocytes
Acta cir. bras
; 33(3): 223-230, Mar. 2018. tab, graf
Article
in En
| LILACS
| ID: biblio-886270
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2).Methods:
Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay.Results:
Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05).Conclusions:
Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.Key words
Full text:
1
Database:
LILACS
Main subject:
Autophagy
/
Cell Hypoxia
/
Cell Survival
/
Apoptosis
/
Microtubule-Associated Proteins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2018
Type:
Article
Affiliation country:
China