Characterization of ndh gene of isoniazid resistant and susceptible Mycobacterium tuberculosis isolates from Brazil
Mem. Inst. Oswaldo Cruz
; 102(1): 59-61, Feb. 2007. tab
Article
in En
| LILACS
| ID: lil-440635
Responsible library:
BR1.1
ABSTRACT
Resistance in Mycobacterium tuberculosis to isoniazid (INH) is caused by mutations in the catalase-peroxidase gene (katG) , and within the inhA promoter and/or in structural gene. A small percentage (~ 10 percent) of INH-resistant strains do not present mutations in both of these loci. Other genes have been associated with INH resistance including the gene encoding for NADH dehydrogenase (ndh) . Here we report the detection of two ndh locus mutations (CGT to TGT change in codon 13 and GTG to GCG change in codon 18) by analyzing 23 INH-resistant and in none of 13 susceptible isolates from Brazilian tuberculosis patients. We also detected two isolates without a mutation in ndh, or any of the other INH resistance-associated loci examined, suggesting the existence of additional, as yet to be described, INH resistance mechanisms.
Full text:
1
Database:
LILACS
Main subject:
Isoniazid
/
Mutation
/
Mycobacterium tuberculosis
/
NADH Dehydrogenase
/
Antitubercular Agents
Limits:
Humans
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
MEDICINA TROPICAL
/
PARASITOLOGIA
Year:
2007
Type:
Article
Affiliation country:
Brazil