Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
Biol. Res
; 39(4): 641-648, 2006. ilus, graf
Article
in En
| LILACS
| ID: lil-456599
Responsible library:
BR1.1
ABSTRACT
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes.
Key words
Full text:
1
Database:
LILACS
Main subject:
Transcription Factors
/
Fungal Proteins
/
Phanerochaete
/
Saccharomyces cerevisiae Proteins
/
DNA-Binding Proteins
Type of study:
Prognostic_studies
Language:
En
Journal:
Biol. Res
Journal subject:
BIOLOGIA
Year:
2006
Type:
Article
/
Project document
Affiliation country:
Chile