Studies on the functional site on staphylococcal enterotoxin A responsible for production of murine gamma interferon.
FEMS Immunol Med Microbiol
; 25(3): 237-44, 1999 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-10459578
ABSTRACT
To identify the functional region(s) associated with induction of gamma interferon on the staphylococcal enterotoxin A molecule, native staphylococcal enterotoxin A molecules and 12 various synthetic peptides corresponding to different regions of entire staphylococcal enterotoxin A were compared to induce gamma interferon production in murine spleen cells. The native staphylococcal enterotoxin A molecule induced gamma interferon production, whereas all of the 12 synthetic peptides did not. Pre-treatment of the murine spleen cells with synthetic peptide A-9 (corresponding to amino acid residues 161-180) significantly inhibited the staphylococcal enterotoxin A-induced gamma interferon production, whereas those with other synthetic peptides did not. When native staphylococcal enterotoxin A was pre-treated with either anti-staphylococcal enterotoxin A serum or anti-peptide sera, anti-staphylococcal enterotoxin A serum and antisera to peptides A-1 (1-20), A-7 (121-140), A-8 (141-160), A-9 (161-180) and A-10 (181-200) inhibited the staphylococcal enterotoxin A-induced gamma interferon production. From these findings, the amino acid residues 161-180 on the staphylococcal enterotoxin A molecule may be an essential region for murine gamma interferon production. Furthermore, the neutralizing epitopes may be also located on regions of amino acid residues 1-20, 121-140, 141-160 and 181-200 on the staphylococcal enterotoxin A molecule.
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Database:
MEDLINE
Main subject:
Staphylococcus aureus
/
Interferon Inducers
/
Interferon-gamma
/
Superantigens
/
Enterotoxins
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
FEMS Immunol Med Microbiol
Journal subject:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
1999
Type:
Article
Affiliation country:
Japan