Protein 4.1N binding to nuclear mitotic apparatus protein in PC12 cells mediates the antiproliferative actions of nerve growth factor.
J Neurosci
; 19(24): 10747-56, 1999 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-10594058
ABSTRACT
Protein 4.1N is a neuronal selective isoform of the erythrocyte membrane cytoskeleton protein 4.1R. In the present study, we demonstrate an interaction between 4.1N and nuclear mitotic apparatus protein (NuMA), a nuclear protein required for mitosis. The binding involves the C-terminal domain of 4.1N. In PC12 cells treatment with nerve growth factor (NGF) elicits translocation of 4. 1N to the nucleus and promotes its association with NuMA. Specific targeting of 4.1N to the nucleus arrests PC12 cells at the G1 phase and produces an aberrant nuclear morphology. Inhibition of 4.1N nuclear translocation prevents the NGF-mediated arrest of cell division, which can be reversed by overexpression of 4.1N. Thus, nuclear 4.1N appears to mediate the antiproliferative actions of NGF by antagonizing the role of NuMA in mitosis.
Full text:
1
Database:
MEDLINE
Main subject:
Neuropeptides
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Nuclear Proteins
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PC12 Cells
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Nerve Growth Factor
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Cytoskeletal Proteins
/
Membrane Proteins
Limits:
Animals
Language:
En
Journal:
J Neurosci
Year:
1999
Type:
Article
Affiliation country:
United States