Yeast Cox11, a protein essential for cytochrome c oxidase assembly, is a Cu(I)-binding protein.
J Biol Chem
; 277(34): 31237-42, 2002 Aug 23.
Article
in En
| MEDLINE
| ID: mdl-12063264
ABSTRACT
Cox11 is a protein essential for respiratory growth and has been implicated in the assembly of the Cu(B) site of cytochrome c oxidase. In the present study, we demonstrate that Cox11 is a copper-binding protein. The soluble C-terminal domain of Cox11 forms a dimer that coordinates one Cu(I) per monomer via three thiolate ligands. The two Cu(I) ions in the dimer exist in a binuclear cluster and appear to be ligated by three conserved Cys residues. Mutation of any of these Cys residues reduces Cu(I) binding and confers respiratory incompetence. Cytochrome c oxidase activity is reduced in these mutants. Thus, the residues important for Cu(I) binding correlate with in vivo function, suggesting that Cu(I) binding is important in Cox11 function.
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Database:
MEDLINE
Main subject:
Carrier Proteins
/
Electron Transport Complex IV
/
Saccharomyces cerevisiae Proteins
/
Membrane Proteins
Language:
En
Journal:
J Biol Chem
Year:
2002
Type:
Article
Affiliation country:
United States