Small molecule LFA-1 antagonists compete with an anti-LFA-1 monoclonal antibody for binding to the CD11a I domain: development of a flow-cytometry-based receptor occupancy assay.

Woska, Joseph R; Last-Barney, Kathleen; Rothlein, Robert; Kroe, Rachel R; Reilly, Patricia L; Jeanfavre, Deborah D; Mainolfi, Elizabeth A; Kelly, Terence A; Caviness, Gary O; Fogal, Steven E; Panzenbeck, Maret J; Kishimoto, Takashi K; Giblin, Patricia A

Woska, Joseph R; Last-Barney, Kathleen; Rothlein, Robert; Kroe, Rachel R; Reilly, Patricia L; Jeanfavre, Deborah D; Mainolfi, Elizabeth A; Kelly, Terence A; Caviness, Gary O; Fogal, Steven E; Panzenbeck, Maret J; Kishimoto, Takashi K; Giblin, Patricia A.

Affiliation

Woska JR; Department of Biology, Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Rd., P.O. Box 368, Ridgefield, CT 06877, USA. jwoska@rdg.boehringer-ingelheim.com

J Immunol Methods ; 277(1-2): 101-15, 2003 Jun 01.

Article in En | MEDLINE | ID: mdl-12799043

Subject(s)

Antibodies, Monoclonal/metabolism; CD11a Antigen/metabolism; Flow Cytometry/methods; Imidazoles/metabolism; Imidazolidines; Lymphocyte Function-Associated Antigen-1/immunology; Animals; Antibodies, Monoclonal/immunology; Antibody Specificity/immunology; Binding, Competitive; CD11a Antigen/immunology; Cell Adhesion Molecules/immunology; Cell Adhesion Molecules/physiology; Humans; Imidazoles/pharmacology; Lymphocyte Function-Associated Antigen-1/metabolism; Lymphocytes/immunology; Lymphocytes/metabolism; Neutrophils/immunology; Neutrophils/metabolism; Pan troglodytes; Receptors, Leukocyte-Adhesion/immunology; Receptors, Leukocyte-Adhesion/physiology; Saimiri

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Database: MEDLINE Main subject: Lymphocyte Function-Associated Antigen-1 / CD11a Antigen / Imidazolidines / Flow Cytometry / Imidazoles / Antibodies, Monoclonal Limits: Animals / Humans Language: En Journal: J Immunol Methods Year: 2003 Type: Article Affiliation country: United States

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