Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12.
J Bacteriol
; 185(20): 6158-70, 2003 Oct.
Article
in En
| MEDLINE
| ID: mdl-14526028
ABSTRACT
Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA levels after exposure to these agents. Real-time reverse transcriptase PCRanalysis served to verify observations made with microarrays, and a chromosomal grpElux operon fusion was employed to specifically monitor the heat shock response. 4-Azaleucine, a competitive inhibitor of leucyl-tRNA synthetase, surprisingly triggered the heat shock response. Administration of mupirocin, an inhibitor of isoleucyl-tRNA synthetase activity, resulted in changes reminiscent of the stringent response. Treatment with kasugamycin and puromycin (targeting ribosomal subunit association as well as its peptidyl-transferase activity) caused accumulation of mRNAs from ribosomal protein operons. Abundant biosynthetic transcripts were often significantly diminished after treatment with any of these agents. Exposure of a relA strain to mupirocin resulted in accumulation of ribosomal protein operon transcripts. However, the relA strain's response to the other inhibitors was quite similar to that of the wild-type strain.
Full text:
1
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Protein Biosynthesis
/
Protein Synthesis Inhibitors
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Escherichia coli Proteins
/
Aminoglycosides
/
Leucine
Language:
En
Journal:
J Bacteriol
Year:
2003
Type:
Article
Affiliation country:
United States