Use of RGS-insensitive Galpha subunits to study endogenous RGS protein action on G-protein modulation of N-type calcium channels in sympathetic neurons.

Ikeda, Stephen R; Jeong, Seong-Woo

Ikeda, Stephen R; Jeong, Seong-Woo.

Affiliation

Ikeda SR; Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Insitutes of Health, Bethesda, Maryland 20892, USA.

Methods Enzymol ; 389: 170-89, 2004.

Article in En | MEDLINE | ID: mdl-15313566

Subject(s)

Calcium Channels, N-Type/physiology; GTP-Binding Protein alpha Subunits, Gi-Go/metabolism; GTP-Binding Proteins/metabolism; Neurons/physiology; RGS Proteins/metabolism; Amino Acid Sequence; Amino Acid Substitution; Animals; Calcium Channels, N-Type/drug effects; Cells, Cultured; Conserved Sequence; GTP-Binding Protein alpha Subunits, Gi-Go/chemistry; GTP-Binding Protein alpha Subunits, Gi-Go/genetics; Humans; Molecular Sequence Data; Neurons/drug effects; Pertussis Toxin/genetics; Pertussis Toxin/pharmacology; Point Mutation; Protein Structure, Tertiary; RGS Proteins/chemistry; RGS Proteins/genetics; Sequence Homology, Amino Acid; Serine/metabolism; Signal Transduction

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Database: MEDLINE Main subject: GTP-Binding Proteins / GTP-Binding Protein alpha Subunits, Gi-Go / Calcium Channels, N-Type / RGS Proteins / Neurons Limits: Animals / Humans Language: En Journal: Methods Enzymol Year: 2004 Type: Article Affiliation country: United States

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