Design and optimization of a recombinant system for large-scale production of the MPT64 antigen from Mycobacterium tuberculosis.

Geisbrecht, Brian V; Nikonenko, Boris; Samala, Rowena; Nakamura, Reiko; Nacy, Carol A; Sacksteder, Katherine A

Geisbrecht, Brian V; Nikonenko, Boris; Samala, Rowena; Nakamura, Reiko; Nacy, Carol A; Sacksteder, Katherine A.

Affiliation

Geisbrecht BV; Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA.

Protein Expr Purif ; 46(1): 64-72, 2006 Mar.

Article in En | MEDLINE | ID: mdl-16216527

Subject(s)

Antigens, Bacterial/genetics; Mycobacterium tuberculosis/genetics; Amino Acid Sequence; Animals; Antigens, Bacterial/chemistry; Antigens, Bacterial/immunology; Antigens, Bacterial/isolation & purification; Base Sequence; DNA Primers; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/genetics; Guinea Pigs; Humans; Molecular Sequence Data; Peptide Fragments/chemistry; Recombinant Proteins/chemistry; Recombinant Proteins/immunology; Recombinant Proteins/isolation & purification; Restriction Mapping; Skin Tests; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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Database: MEDLINE Main subject: Mycobacterium tuberculosis / Antigens, Bacterial Type of study: Guideline Limits: Animals / Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2006 Type: Article Affiliation country: United States

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