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Crystallization and preliminary X-ray diffraction analysis of a flavoenzyme amine dehydrogenase/oxidase from Pyrococcus furiosus DSM 3638.
Monaghan, Phillip J; Leys, David; Scrutton, Nigel S.
Affiliation
  • Monaghan PJ; Department of Biochemistry, University of Leicester, Leicester LE1 7RH, England.
Article in En | MEDLINE | ID: mdl-16511149
ABSTRACT
A flavoprotein amine dehydrogenase/oxidase with subunit molecular weights of 54.8 kDa (alpha-subunit) and 42.4 kDa (beta-subunit) and specificity for L-proline was cloned from the genomic DNA of the hyperthermophilic marine archaeon Pyrococcus furiosus DSM 3638. The enzyme was overexpressed in Escherichia coli and purified to homogeneity. The enzyme was crystallized using the sitting-drop vapour-diffusion technique. Diffraction data from two different crystal forms were collected to 3.3 and 3.6 A, respectively, using synchrotron radiation. Both crystals belonged to space group P1, with unit-cell parameters a = 91.3, b = 136.3, c = 203.8 A, alpha = 94.5, beta = 99.4, gamma = 102.7 degrees and a = 93.7, b = 116.3, c = 126.9 A, alpha = 97.3, beta = 99.9, gamma = 104.6 degrees.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Pyrococcus furiosus / Monoamine Oxidase Language: En Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Year: 2005 Type: Article Affiliation country: United kingdom

Full text: 1 Database: MEDLINE Main subject: Pyrococcus furiosus / Monoamine Oxidase Language: En Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Year: 2005 Type: Article Affiliation country: United kingdom