Levels of detection of Cryptosporidium oocysts in mussels (Mytilus galloprovincialis) by IFA and PCR methods.
Vet Parasitol
; 141(1-2): 60-5, 2006 Oct 10.
Article
in En
| MEDLINE
| ID: mdl-16720079
ABSTRACT
Cryptosporidium spp. are monoxenous protozoan parasites that cause gastrointestinal diseases in humans and animals. Shellfish harvesting areas can become contaminated by the infectious stage of the parasite and humans are therefore at risk of infection either by consumption of shellfish, or by taking part in recreational activities in these areas. In the present study we determined the levels of detection, by IFA and PCR techniques, of Cryptosporidium oocysts in mussels experimentally contaminated with a theoretical number of oocysts. There was a significant correlation between the results obtained by both techniques (P<0.05). IFA and PCR were also applied to a total of 222 samples of mussels (Mytilus galloprovincialis) destined for human consumption. In the naturally contaminated samples, we detected a 31.1% of contamination and only Cryptosporidium parvum (previously denominated C. parvum genotype II) was identified.
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Database:
MEDLINE
Main subject:
Shellfish
/
Polymerase Chain Reaction
/
Bivalvia
/
Fluorescent Antibody Technique, Indirect
/
Cryptosporidium
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Vet Parasitol
Year:
2006
Type:
Article
Affiliation country:
Spain