The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements.
Nat Biotechnol
; 24(9): 1151-61, 2006 Sep.
Article
in En
| MEDLINE
| ID: mdl-16964229
ABSTRACT
Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues. Expression data on four titration pools from two distinct reference RNA samples were generated at multiple test sites using a variety of microarray-based and alternative technology platforms. Here we describe the experimental design and probe mapping efforts behind the MAQC project. We show intraplatform consistency across test sites as well as a high level of interplatform concordance in terms of genes identified as differentially expressed. This study provides a resource that represents an important first step toward establishing a framework for the use of microarrays in clinical and regulatory settings.
Full text:
1
Database:
MEDLINE
Main subject:
Quality Assurance, Health Care
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Country/Region as subject:
America do norte
Language:
En
Journal:
Nat Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2006
Type:
Article