Contribution of yeast artificial chromosome-based physical maps to the final assembly of the Trypanosoma cruzi genome.

ABSTRACT

This chapter describes the methodology used both in performing the electrophoretic karyotype of the protozoan parasite Trypanosoma cruzi and mapping the genetic markers of the chromosomal bands, the construction of chromosome-specific YAC contigs, and their use to assign a chromosomal location to whole genome shotgun sequences.