Somatic hypermutation: activation-induced deaminase for C/G followed by polymerase eta for A/T.
J Exp Med
; 204(1): 7-10, 2007 Jan 22.
Article
in En
| MEDLINE
| ID: mdl-17190841
ABSTRACT
Somatic hypermutation (SHM) introduces nucleotide substitutions into immunoglobulin variable (Ig V) region genes at all four bases, but the mutations at C/G and A/T pairs are achieved by distinct mechanisms. Mutations at C/G pairs are a direct consequence of the C-->U deamination catalyzed by activation-induced deaminase (AID). Mutations at A/T pairs, however, require a second mutagenic process that occurs during patch repair of the AID-generated U/G mismatch. Several DNA polymerases have been proposed to play a role in SHM, but accumulating evidence indicates that the mutations at A/T are overwhelmingly achieved by recruitment of DNA polymerase eta.
Full text:
1
Database:
MEDLINE
Main subject:
Cytidine Deaminase
/
Somatic Hypermutation, Immunoglobulin
/
DNA-Directed DNA Polymerase
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
J Exp Med
Year:
2007
Type:
Article
Affiliation country:
United kingdom