Switching constant domains enhances agonist activities of antibodies to a thrombopoietin receptor.
Nat Biotechnol
; 26(2): 209-11, 2008 Feb.
Article
in En
| MEDLINE
| ID: mdl-18157117
ABSTRACT
We enhanced the activities of two agonist antibodies specific for the thrombopoietin receptor (c-MPL) by switching domains within their constant regions to those of different antibody isotypes. Our results suggest the importance of the hinge region in modulating agonist activity. The antibodies' thrombopoietin-like activity in vitro and in vivo, as well as the desirable pharmacokinetic profile conferred by retaining the whole-IgG structure, suggests that they provide a valuable option for treating thrombocytopenia.
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Database:
MEDLINE
Main subject:
Protein Engineering
/
Genetic Enhancement
/
Receptors, Thrombopoietin
/
Antibodies, Monoclonal
Language:
En
Journal:
Nat Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2008
Type:
Article
Affiliation country:
Japan