Enhanced protein expression in mammalian cells using engineered SUMO fusions: secreted phospholipase A2.

Peroutka, Raymond J; Elshourbagy, Nabil; Piech, Tara; Butt, Tauseef R

Peroutka, Raymond J; Elshourbagy, Nabil; Piech, Tara; Butt, Tauseef R.

Affiliation

Peroutka RJ; LifeSensors, Inc., Malvern, Pennsylvania 19355, USA.

Protein Sci ; 17(9): 1586-95, 2008 Sep.

Article in En | MEDLINE | ID: mdl-18539905

Subject(s)

Group X Phospholipases A2/metabolism; Phospholipases A2/metabolism; Protein Engineering/methods; Small Ubiquitin-Related Modifier Proteins/metabolism; Animals; CHO Cells; Cell Line; Cricetinae; Cricetulus; Group X Phospholipases A2/genetics; Histidine/chemistry; Humans; Kidney/cytology; Mice; Phospholipases A2/genetics; Protein Folding; Protein Processing, Post-Translational; Recombinant Fusion Proteins/metabolism; Small Ubiquitin-Related Modifier Proteins/genetics; Solubility

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Full text: 1 Database: MEDLINE Main subject: Protein Engineering / Small Ubiquitin-Related Modifier Proteins / Phospholipases A2 / Group X Phospholipases A2 Limits: Animals / Humans Language: En Journal: Protein Sci Journal subject: BIOQUIMICA Year: 2008 Type: Article Affiliation country: United States

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