A refinery sludge deposition site: presence of nahH and alkJ genes and crude oil biodegradation ability of bacterial isolates.
Biotechnol Lett
; 30(12): 2105-10, 2008 Dec.
Article
in En
| MEDLINE
| ID: mdl-18688575
ABSTRACT
204 bacterial isolates from four Greek refinery sludge deposition sites were investigated for the presence of nahH and alkJ genes encoding key enzymes of both aromatic and aliphatic hydrocarbon degradation pathways by PCR and DNA hybridisation. Members of Pseudomonas, Acinetobacter, Bacillus, Rhodococcus and Arthrobacter play important role in bioremediation processes in sandy/loam soil contaminated with oil and nahH and alkJ genes were present in the 73% of the isolates. Consortia of bacterial isolates that were used for biodegradation of aliphatic and aromatic hydrocarbons in crude oil using liquid cultures exhibited rates from 35% to 48% within 10 days of incubation.
Full text:
1
Database:
MEDLINE
Main subject:
Soil Microbiology
/
Soil Pollutants
/
Bacteria, Aerobic
/
Biodegradation, Environmental
/
Petroleum
/
Alcohol Oxidoreductases
/
Catechol 2,3-Dioxygenase
Language:
En
Journal:
Biotechnol Lett
Year:
2008
Type:
Article
Affiliation country:
Greece