Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6.
Biochim Biophys Acta
; 1090(1): 81-5, 1991 Aug 27.
Article
in En
| MEDLINE
| ID: mdl-1883845
ABSTRACT
A clone of about 1 kb has been isolated from a human brain cDNA library. The clone possesses a 151 amino acid open reading frame that exhibits 72% amino acid identity with the E2 ubiquitin-conjugating enzyme encoded by the RAD6 gene of Saccharomyces cerevisiae. A 90% amino acid identity was observed in a central sequence surrounding a cysteine, which most likely contributes the sulfhydryl group involved in the formation of the ubiquitin-E2 thiolester linkage. Northern hybridization analyses have identified a poly(A)-containing mRNA of about 1 kb encoding the E2-like sequence in human CEM lymphoblastoid and HeLa cells, Novikoff rat hepatoma cells and S49 mouse leukemia cells. Southern hybridization analyses indicate the presence of a single gene encoding this sequence in both human cell lines, but of two or more related genes in the rodent cell lines.
Search on Google
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Sequence Homology, Nucleic Acid
/
Carrier Proteins
/
Ubiquitin-Conjugating Enzymes
/
Ligases
Limits:
Animals
/
Humans
Language:
En
Journal:
Biochim Biophys Acta
Year:
1991
Type:
Article