Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia.
Leuk Res
; 33(8): 1082-8, 2009 Aug.
Article
in En
| MEDLINE
| ID: mdl-19081626
ABSTRACT
Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL. The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation.
Full text:
1
Database:
MEDLINE
Main subject:
Biomarkers, Tumor
/
Oncogene Proteins, Fusion
/
Polymerase Chain Reaction
/
Core Binding Factor Alpha 2 Subunit
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Type of study:
Prognostic_studies
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
En
Journal:
Leuk Res
Year:
2009
Type:
Article
Affiliation country:
Germany