Closing gaps in the human genome using sequencing by synthesis.
Genome Biol
; 10(6): R60, 2009.
Article
in En
| MEDLINE
| ID: mdl-19490611
ABSTRACT
The most recent release of the finished human genome contains 260 euchromatic gaps (excluding chromosome Y). Recent work has helped explain a large number of these unresolved regions as 'structural' in nature. Another class of gaps is likely to be refractory to clone-based approaches, and cannot be approached in ways previously described. We present an approach for closing these gaps using 454 sequencing. As a proof of principle, we closed all three remaining non-structural gaps in chromosome 15.
Full text:
1
Database:
MEDLINE
Main subject:
Genome, Human
/
Sequence Analysis, DNA
Limits:
Humans
Language:
En
Journal:
Genome Biol
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2009
Type:
Article
Affiliation country:
United States