Clostridium botulinum toxin A inhibits contractility in pregnant human myometrium in vitro.
Reprod Sci
; 16(10): 1001-4, 2009 Oct.
Article
in En
| MEDLINE
| ID: mdl-19602724
ABSTRACT
Studies were undertaken to evaluate the effect of Botulinum neurotoxin type-A (BoNTA) preparation on oxytocin-induced contractions of pregnant human myometrium in vitro. Human myometrial tissue was exposed to increasing concentrations (1-50 000 U/mL) of BoNT/A. Isometric contractions were measured using a force displacement transducer. The cumulative effect of BoNT/A on myometrial activity (time to half relaxation [TTR50], frequency, and amplitude) was evaluated. The frequency of myometrial contractions was depressed by 40% from baseline (P < .05) and relaxation time was increased by 30% (P < .05) from baseline within a narrow range of concentrations. There was no significant difference in amplitude. The observed effects were rapidly reversed after complete wash out of the tissue. BoNT/A or its analogues with more specific tissue affinity may be of value as future agents for prevention of unwanted uterine contractile activity associated with preterm labor and fetal surgery.
Full text:
1
Database:
MEDLINE
Main subject:
Uterine Contraction
/
Botulinum Toxins, Type A
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Reprod Sci
Journal subject:
MEDICINA REPRODUTIVA
Year:
2009
Type:
Article
Affiliation country:
United States