Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cells.
Nat Cell Biol
; 11(9): 1069-80, 2009 Sep.
Article
in En
| MEDLINE
| ID: mdl-19701196
ABSTRACT
Microtubules are indispensable for Golgi complex assembly and maintenance, which are integral parts of cytoplasm organization during interphase in mammalian cells. Here, we show that two discrete microtubule subsets drive two distinct, yet simultaneous, stages of Golgi assembly. In addition to the radial centrosomal microtubule array, which positions the Golgi in the centre of the cell, we have identified a role for microtubules that form at the Golgi membranes in a manner dependent on the microtubule regulators CLASPs. These Golgi-derived microtubules draw Golgi ministacks together in tangential fashion and are crucial for establishing continuity and proper morphology of the Golgi complex. We propose that specialized functions of these two microtubule arrays arise from their specific geometries. Further, we demonstrate that directional post-Golgi trafficking and cell migration depend on Golgi-associated CLASPs, suggesting that correct organization of the Golgi complex by microtubules is essential for cell polarization and motility.
Full text:
1
Database:
MEDLINE
Main subject:
Cell Movement
/
Cell Polarity
/
Golgi Apparatus
/
Microtubule-Associated Proteins
/
Microtubules
Limits:
Humans
Language:
En
Journal:
Nat Cell Biol
Year:
2009
Type:
Article
Affiliation country:
United States