Transcriptional regulation of Tlr11 gene expression in epithelial cells.
J Biol Chem
; 284(48): 33088-96, 2009 Nov 27.
Article
in En
| MEDLINE
| ID: mdl-19801549
ABSTRACT
As sensors of invading microorganisms, Toll-like receptors (TLRs) are expressed not only on macrophages and dendritic cells (DCs) but also on epithelial cells. In the TLR family, Tlr11 appears to have the unique feature in that it is expressed primarily on epithelial cells, although it is also expressed on DCs and macrophages. Here, we demonstrate that transcription of the Tlr11 gene is regulated through two cis-acting elements, one Ets-binding site and one interferon regulatory factor (IRF)-binding site. The Ets element interacts with the epithelium-specific transcription factors, ESE-1 and ESE-3, and the IRF motif interacts with IRF-8. Thus, Tlr11 expression on epithelial cells is regulated by the transcription factors that are presumably distinct from transcription factors that regulate the expression of TLRs in innate immune cells such as macrophages and DCs. Our results imply that the distinctive transcription regulatory machinery for TLRs on epithelium may represent a promising new avenue for the development of epithelia-specific therapeutic interventions.
Full text:
1
Database:
MEDLINE
Main subject:
Regulatory Sequences, Nucleic Acid
/
Gene Expression Regulation
/
Epithelial Cells
/
Toll-Like Receptors
Limits:
Animals
/
Humans
Language:
En
Journal:
J Biol Chem
Year:
2009
Type:
Article
Affiliation country:
United States