Designed surface with tunable IgG density as an in vitro model for immune complex mediated stimulation of leukocytes.
Langmuir
; 26(5): 3493-7, 2010 Mar 02.
Article
in En
| MEDLINE
| ID: mdl-20000861
ABSTRACT
We present the design of an in vitro model for immune-complex-mediated stimulation of leukocytes and its functional characteristics with respect to monocyte adhesion. The model was based on the orientation-controlled immobilization of a humanized IgG1 monoclonal antibody (rituximab) via its interaction with a biotinylated peptide epitope derived from the CD20 marker. The peptide was linked to neutravidin covalently attached to a mixed self-assembled monolayer of carboxyl- and methoxy-terminated oligo(ethylene glycol) alkane thiolates on gold. The surface adhesion propensity of human monocytes (cell line U937) was highly dependent on the lateral IgG density and indicated that there exists a distance between IgG-Fc on the surface where interactions with Fc gamma receptors are optimal. This well-defined platform allows for a careful control of the size and orientation of artificial IgG immune complexes, it is easily made compatible with, for example, cellular imaging, and it will become useful for in vitro studies on the importance of Fc gamma receptor interactions in chronic immune-mediated diseases.
Full text:
1
Database:
MEDLINE
Main subject:
Immunoglobulin G
/
Models, Immunological
/
Leukocytes
/
Antigen-Antibody Complex
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Langmuir
Journal subject:
QUIMICA
Year:
2010
Type:
Article
Affiliation country:
Sweden