Immunomodulatory strategies prevent the development of autoimmune emphysema.
Respir Res
; 11: 179, 2010 Dec 16.
Article
in En
| MEDLINE
| ID: mdl-21162738
ABSTRACT
BACKGROUND:
The presence of anti-endothelial cell antibodies and pathogenic T cells may reflect an autoimmune component in the pathogenesis of emphysema. Whether immune modulatory strategies can protect against the development of emphysema is not known.METHODS:
Sprague Dawley rats were immunized with human umbilical vein endothelial cells (HUVEC) to induce autoimmune emphysema and treated with intrathymic HUVEC-injection and pristane. Measurements of alveolar airspace enlargement, cytokine levels, immuno histochemical, western blot analysis, and T cell repertoire of the lung tissue were performed.RESULTS:
The immunomodulatory strategies protected lungs against cell death as demonstrated by reduced numbers of TUNEL and active caspase-3 positive cells and reduced levels of active caspase-3, when compared with lungs from HUVEC-immunized rats. Immunomodulatory strategies also suppressed anti-endothelial antibody production and preserved CNTF, IL-1alpha and VEGF levels. The immune deviation effects of the intrathymic HUVEC-injection were associated with an expansion of CD4+CD25+Foxp3+ regulatory T cells. Pristane treatment decreased the proportion of T cells expressing receptor beta-chain, Vß16.1 in the lung tissue.CONCLUSIONS:
Our data demonstrate that interventions classically employed to induce central T cell tolerance (thymic inoculation of antigen) or to activate innate immune responses (pristane treatment) can prevent the development of autoimmune emphysema.
Full text:
1
Database:
MEDLINE
Main subject:
Autoimmune Diseases
/
Autoimmunity
/
Cytokines
/
Emphysema
/
Immunomodulation
Limits:
Animals
Language:
En
Journal:
Respir Res
Year:
2010
Type:
Article
Affiliation country:
United States