The mitochondrial DNA 4,977-bp deletion and its implication in copy number alteration in colorectal cancer.
BMC Med Genet
; 12: 8, 2011 Jan 13.
Article
in En
| MEDLINE
| ID: mdl-21232124
ABSTRACT
BACKGROUND:
qualitative and quantitative changes in human mitochondrial DNA (mtDNA) have been implicated in various cancer types. A 4,977 bp deletion in the major arch of the mitochondrial genome is one of the most common mutations associated with a variety of human diseases and aging.METHODS:
we conducted a comprehensive study on clinical features and mtDNA of 104 colorectal cancer patients in the Wenzhou area of China. In particular, using a quantitative real time PCR method, we analyzed the 4,977 bp deletion and mtDNA content in tumor tissues and paired non-tumor areas from these patients.RESULTS:
we found that the 4,977 bp deletion was more likely to be present in patients of younger age (≤65 years, p = 0.027). In patients with the 4,977 bp deletion, the deletion level decreased as the cancer stage advanced (p = 0.031). Moreover, mtDNA copy number in tumor tissues of patients with this deletion increased, both compared with that in adjacent non-tumor tissues and with in tumors of patients without the deletion. Such mtDNA content increase correlated with the levels of the 4,977 bp deletion and with cancer stage (p < 0.001).CONCLUSIONS:
our study indicates that the mtDNA 4,977 bp deletion may play a role in the early stage of colorectal cancer, and it is also implicated in alteration of mtDNA content in cancer cells.
Full text:
1
Database:
MEDLINE
Main subject:
DNA, Mitochondrial
/
Colorectal Neoplasms
/
Sequence Deletion
/
DNA Copy Number Variations
Type of study:
Incidence_studies
/
Prognostic_studies
/
Qualitative_research
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Asia
Language:
En
Journal:
BMC Med Genet
Journal subject:
GENETICA MEDICA
Year:
2011
Type:
Article