Separation and purification of Escherichia coli-expressed human thymosin-&#945;1 using affinity chromatography and high-performance liquid chromatography.

Zhang, Hong-Ying; Chen, Pei-Fu; Xu, Jia-Ming; Dai, Quan-Min; Xu, Feng; Han, Qing-Wang; Wang, Jian-Jun; Jin, Hei-Ying

Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography.

Zhang, Hong-Ying; Chen, Pei-Fu; Xu, Jia-Ming; Dai, Quan-Min; Xu, Feng; Han, Qing-Wang; Wang, Jian-Jun; Jin, Hei-Ying.

Affiliation

Zhang HY; Nanjing Center for Disease Control and Prevention, Nanjing 210003, China.

Protein Expr Purif ; 77(2): 140-5, 2011 Jun.

Article in En | MEDLINE | ID: mdl-21256964

Subject(s)

Chromatography, Affinity/methods; Chromatography, High Pressure Liquid/methods; Recombinant Fusion Proteins/isolation & purification; Thymosin/analogs & derivatives; Cloning, Molecular; Escherichia coli; Gene Expression; Histidine/metabolism; Humans; Isoelectric Focusing; Isopropyl Thiogalactoside/metabolism; Oligopeptides/metabolism; Protein Denaturation; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Reproducibility of Results; Solubility; Thymalfasin; Thymosin/genetics; Thymosin/isolation & purification; Thymosin/metabolism

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Full text: 1 Database: MEDLINE Main subject: Thymosin / Recombinant Fusion Proteins / Chromatography, Affinity / Chromatography, High Pressure Liquid Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2011 Type: Article Affiliation country: China

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