Subtypes of voltage-sensitive calcium channels in cultured rat brain neurons.
Neurosci Lett
; 115(2-3): 300-6, 1990 Jul 31.
Article
in En
| MEDLINE
| ID: mdl-2172873
Subtypes of voltage-sensitive calcium channels have been investigated in cultured rat brain neurons using two classes of specific probes, dihydropyridine compounds and omega-conotoxin. Membranes prepared from cultured neurons contain specific binding sites for [3H]PN200-110, a dihydropyridine antagonist, and for 125I-omega-conotoxin with a stoichiometry of about 1:1. A depolarization induced 45Ca2+ influx into intact brain neurons was partially inhibited by a dihydropyridine antagonist, nifedipine and stimulated by a dihydropyridine agonist, Bay K8644. This dihydropyridine sensitive 45Ca2+ flux was insensitive to omega-conotoxin at concentrations which saturate the specific toxin binding sites indicating that in cultured brain neurons, dihydropyridine-sensitive calcium channels are not sensitive to omega-conotoxin.
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Database:
MEDLINE
Main subject:
Oxadiazoles
/
Peptides, Cyclic
/
Brain
/
Calcium Channels
/
Calcium
/
Omega-Conotoxins
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Neurosci Lett
Year:
1990
Type:
Article
Affiliation country:
France