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Subtypes of voltage-sensitive calcium channels in cultured rat brain neurons.
Martin-Moutot, N; Seagar, M; Couraud, F.
Affiliation
  • Martin-Moutot N; Laboratoire de Biochimie, INSERM U 172, CNRS URA 1179, Faculté de Médecine Nord, Marseilles, France.
Neurosci Lett ; 115(2-3): 300-6, 1990 Jul 31.
Article in En | MEDLINE | ID: mdl-2172873
Subtypes of voltage-sensitive calcium channels have been investigated in cultured rat brain neurons using two classes of specific probes, dihydropyridine compounds and omega-conotoxin. Membranes prepared from cultured neurons contain specific binding sites for [3H]PN200-110, a dihydropyridine antagonist, and for 125I-omega-conotoxin with a stoichiometry of about 1:1. A depolarization induced 45Ca2+ influx into intact brain neurons was partially inhibited by a dihydropyridine antagonist, nifedipine and stimulated by a dihydropyridine agonist, Bay K8644. This dihydropyridine sensitive 45Ca2+ flux was insensitive to omega-conotoxin at concentrations which saturate the specific toxin binding sites indicating that in cultured brain neurons, dihydropyridine-sensitive calcium channels are not sensitive to omega-conotoxin.
Subject(s)
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Database: MEDLINE Main subject: Oxadiazoles / Peptides, Cyclic / Brain / Calcium Channels / Calcium / Omega-Conotoxins Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Neurosci Lett Year: 1990 Type: Article Affiliation country: France
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Database: MEDLINE Main subject: Oxadiazoles / Peptides, Cyclic / Brain / Calcium Channels / Calcium / Omega-Conotoxins Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Neurosci Lett Year: 1990 Type: Article Affiliation country: France