Lipid nanoparticle purification by spin centrifugation-dialysis (SCD): a facile and high-throughput approach for small scale preparation of siRNA-lipid complexes.
Int J Pharm
; 420(1): 118-21, 2011 Nov 25.
Article
in En
| MEDLINE
| ID: mdl-21893179
ABSTRACT
This paper describes the use of spin centrifugation-dialysis (SCD) for small-scale concentration/purification of siRNA-lipid complexes designed for use as therapeutic agents for gene silencing. SCD consists of a two-step method for concentration, filtration and buffer exchange of lipid nanoparticles (LNP) to provide a homogeneous preparation suitable for injection. Here, we compare SCD with the more traditionally used tangential flow filtration (TFF), and demonstrate the physicochemical and biological comparability of LNPs produced with both methods. TFF is a highly scalable method used in both developmental and production applications, but is limited in terms of miniaturization. In contrast to TFF, SCD is faster, less expensive, and requires less oversight for assembling LNPs for small-scale applications, such as target screening both in vitro and in vivo. The finding that SCD is a viable method for filtering LNPs in a manner similar to TFF, producing particles with comparable properties and biological activity, is significant given the complexity and sensitivity of LNPs to processing conditions.
Full text:
1
Database:
MEDLINE
Main subject:
Centrifugation
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RNA, Small Interfering
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RNA Interference
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Dialysis
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Nanoparticles
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High-Throughput Screening Assays
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Lipids
Limits:
Animals
Language:
En
Journal:
Int J Pharm
Year:
2011
Type:
Article
Affiliation country:
United States