Antigen-specific transfer of functional programmed death ligand 1 from human APCs onto CD8+ T cells via trogocytosis.
J Immunol
; 188(2): 744-52, 2012 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-22174448
ABSTRACT
Upon specific interaction with APCs, T cells capture membrane fragments and surface molecules in a process termed trogocytosis. In this study, we demonstrate that human Ag-specific CD8(+) T cells acquire the coinhibitory molecule programmed death ligand 1 (PD-L1) from mature dendritic cells (mDC) and tumor cells in an Ag-specific manner. Immature dendritic cells were less effective in transferring surface molecules onto CD8(+) T cells than mDCs. Interestingly, trogocytosis of PD-L1 requires cell-cell contact and cannot be induced by uptake of soluble proteins obtained from mDC lysates. The transfer process is impaired by inhibition of vacuolar ATPases in T cells as well as by fixation of dendritic cells. Of importance, CD8(+) T cells that acquired PD-L1 complexes were able to induce apoptosis of neighboring programmed death 1-expressing CD8(+) T cells. In summary, our data demonstrate that human CD8(+) T cells take up functionally active PD-L1 from APCs in an Ag-specific fashion, leading to fratricide of programmed death 1-expressing, neighboring T cells. The transfer of functionally active coinhibitory molecules from APCs onto human CD8(+) T cells could have a regulatory role in immune responses.
Full text:
1
Database:
MEDLINE
Main subject:
Cell Communication
/
Antigen Presentation
/
CD8-Positive T-Lymphocytes
/
Epitopes, T-Lymphocyte
/
B7-H1 Antigen
/
Antigen-Presenting Cells
Limits:
Humans
Language:
En
Journal:
J Immunol
Year:
2012
Type:
Article
Affiliation country:
Germany