Secretion from bacterial versus mammalian cells yields a recombinant scFv with variable folding properties.

Vendel, Michelle C; Favis, Michael; Snyder, William B; Huang, Flora; Capili, Allan D; Dong, Jianying; Glaser, Scott M; Miller, Brian R; Demarest, Stephen J

Vendel, Michelle C; Favis, Michael; Snyder, William B; Huang, Flora; Capili, Allan D; Dong, Jianying; Glaser, Scott M; Miller, Brian R; Demarest, Stephen J.

Affiliation

Vendel MC; Biogen Idec, 14 Cambridge Center, Cambridge, MA 02142, USA.

Arch Biochem Biophys ; 526(2): 188-93, 2012 Oct 15.

Article in En | MEDLINE | ID: mdl-22230329

Subject(s)

Antibodies, Bispecific/chemistry; Antibodies, Bispecific/genetics; Cloning, Molecular/methods; Single-Chain Antibodies/chemistry; Single-Chain Antibodies/genetics; Animals; CHO Cells; Cricetinae; Escherichia coli/genetics; Humans; Immunoglobulin G/chemistry; Immunoglobulin G/genetics; Models, Molecular; Protein Engineering/methods; Protein Folding; Protein Stability; Protein Structure, Secondary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Solubility

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Full text: 1 Database: MEDLINE Main subject: Cloning, Molecular / Antibodies, Bispecific / Single-Chain Antibodies Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Arch Biochem Biophys Year: 2012 Type: Article Affiliation country: United States

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