Automated workflow for preparation of cDNA for cap analysis of gene expression on a single molecule sequencer.

Itoh, Masayoshi; Kojima, Miki; Nagao-Sato, Sayaka; Saijo, Eri; Lassmann, Timo; Kanamori-Katayama, Mutsumi; Kaiho, Ai; Lizio, Marina; Kawaji, Hideya; Carninci, Piero; Forrest, Alistair R R; Hayashizaki, Yoshihide

Itoh, Masayoshi; Kojima, Miki; Nagao-Sato, Sayaka; Saijo, Eri; Lassmann, Timo; Kanamori-Katayama, Mutsumi; Kaiho, Ai; Lizio, Marina; Kawaji, Hideya; Carninci, Piero; Forrest, Alistair R R; Hayashizaki, Yoshihide.

Affiliation

Itoh M; Omics Science Center, RIKEN Yokohama Institute, Tsurumi-ku, Yokohama, Kanagawa, Japan. maitoh@riken.jp

PLoS One ; 7(1): e30809, 2012.

Article in En | MEDLINE | ID: mdl-22303458

Subject(s)

DNA, Complementary/metabolism; Gene Expression Regulation; Sequence Analysis, DNA/instrumentation; Sequence Analysis, DNA/methods; Workflow; Animals; Automation; Base Sequence; DNA, Complementary/genetics; Gene Library; Genome, Human/genetics; Humans; Mice; Reproducibility of Results

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Full text: 1 Database: MEDLINE Main subject: Gene Expression Regulation / Sequence Analysis, DNA / DNA, Complementary / Workflow Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2012 Type: Article Affiliation country: Japan

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