A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin &#945; as fusion tag.

Nakagawa, Chika; Nishimura, Shigenori; Senda-Murata, Kaori; Sugimoto, Kenji

A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin α as fusion tag.

Nakagawa, Chika; Nishimura, Shigenori; Senda-Murata, Kaori; Sugimoto, Kenji.

Affiliation

Nakagawa C; Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. cnakagawa@bioinfo.osakafu-u.ac.jp

Biosci Biotechnol Biochem ; 76(2): 388-90, 2012.

Article in En | MEDLINE | ID: mdl-22313767

Subject(s)

Cytological Techniques/methods; Luminescent Proteins; Protein Multimerization; alpha Karyopherins; Animals; Cells/chemistry; Green Fluorescent Proteins; Hydrophobic and Hydrophilic Interactions; Methods; Mutation, Missense; Recombinant Fusion Proteins; alpha Karyopherins/genetics

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Database: MEDLINE Main subject: Cytological Techniques / Alpha Karyopherins / Protein Multimerization / Luminescent Proteins Limits: Animals Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2012 Type: Article Affiliation country: Japan

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