CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA.
J Infect Dis
; 205(7): 1095-9, 2012 Apr 01.
Article
in En
| MEDLINE
| ID: mdl-22357696
ABSTRACT
We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.
Full text:
1
Database:
MEDLINE
Main subject:
Sarcoma, Kaposi
/
DNA, Viral
/
DNA-Cytosine Methylases
/
Herpesvirus 8, Human
/
Lymphoma, Primary Effusion
Limits:
Humans
Language:
En
Journal:
J Infect Dis
Year:
2012
Type:
Article
Affiliation country:
United States