Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential.
Drug Test Anal
; 4(9): 733-8, 2012 Sep.
Article
in En
| MEDLINE
| ID: mdl-22962027
ABSTRACT
The formulation TB-500 is suspected to be used as doping agent in sport. This work describes the detection and the identification of the N-terminal acetylated 17-23 fragment of human thymosin beta 4 (Ac-LKKTETQ) in TB-500 by means of high-performance liquid chromatography/high resolution mass spectrometry using an Orbitrap Exactive benchtop mass spectrometer. Ac-LKKTETQ was also synthesized by solid-phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high-performance liquid chromatography/low resolution triple-quadrupole mass spectrometry was suggested.
Full text:
1
Database:
MEDLINE
Main subject:
Thymosin
/
Substance Abuse Detection
/
Tandem Mass Spectrometry
/
Performance-Enhancing Substances
Limits:
Humans
Language:
En
Journal:
Drug Test Anal
Journal subject:
FARMACOLOGIA
Year:
2012
Type:
Article
Affiliation country:
Belgium