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Epithelial expression of interleukin-37b in inflammatory bowel disease.
Imaeda, H; Takahashi, K; Fujimoto, T; Kasumi, E; Ban, H; Bamba, S; Sonoda, H; Shimizu, T; Fujiyama, Y; Andoh, A.
Affiliation
  • Imaeda H; Department of Medicine, Shiga University of Medical Science, Otsu, Japan.
Clin Exp Immunol ; 172(3): 410-6, 2013 Jun.
Article in En | MEDLINE | ID: mdl-23600829
ABSTRACT
Interleukin (IL)-37 is a member of the IL-1 cytokine family. We investigated IL-37b expression in the inflamed mucosa of inflammatory bowel disease (IBD) patients. Furthermore, we analysed IL-37b expression in human colonic epithelial cells. The human colonic epithelial cell line T84 and human colonic subepithelial myofibroblasts (SEMFs) were used. IL-37b expression in the IBD mucosa was evaluated by immunohistochemistry. IL-37b mRNA and protein expression were determined by real time-polymerase chain reaction (PCR) and Western blotting, respectively. IL-37b was not detected in the normal colonic mucosa. In the inflamed mucosa of IBD patients, epithelial IL-37b expression was increased markedly. In ulcerative colitis (UC) and Crohn's disease (CD) patients, IL-37b expression was enhanced in the affected mucosa. In the intestinal epithelial cell line T84, the expression of IL-37b mRNA and protein was enhanced by tumour necrosis factor (TNF)-α. This IL-37b induction by TNF-α was mediated by nuclear factor (NF)-κB and activator protein (AP)-1 activation. Furthermore, IL-37b inhibited TNF-α-induced interferon-γ-inducible protein (IP)-10 expression significantly in human colonic SEMFs. Epithelial IL-37b expression was increased in IBD patients, especially UC patients. IL-37b may be involved in the pathophysiology of IBD as an anti-inflammatory cytokine and an inhibitor of both innate and acquired immune responses.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Inflammatory Bowel Diseases / Interleukin-1 Type of study: Observational_studies Language: En Journal: Clin Exp Immunol Year: 2013 Type: Article Affiliation country: Japan

Full text: 1 Database: MEDLINE Main subject: Inflammatory Bowel Diseases / Interleukin-1 Type of study: Observational_studies Language: En Journal: Clin Exp Immunol Year: 2013 Type: Article Affiliation country: Japan