The malaria vaccine candidate GMZ2 elicits functional antibodies in individuals from malaria endemic and non-endemic areas.
J Infect Dis
; 208(3): 479-88, 2013 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-23624363
ABSTRACT
BACKGROUND:
GMZ2 is a hybrid protein consisting of the N-terminal region of the glutamate-rich protein fused in frame to the C-terminal region of merozoite surface protein 3 (MSP3). GMZ2 formulated in Al(OH)3 has been tested in 3 published phase 1 clinical trials. The GMZ2/alum formulation showed good safety, tolerability, and immunogenicity, but whether antibodies elicited by vaccination are functional is not known.METHODS:
Serum samples prior to vaccination and 4 weeks after the last vaccination from the 3 clinical trials were used to perform a comparative assessment of biological activity against Plasmodium falciparum.RESULTS:
We showed that the maximum level of immunoglobulin G (IgG) antibodies obtained by GMZ2 vaccination is independent of ethnicity, time under malaria-exposure, and vaccine dose and that GMZ2 elicits high levels of functionally active IgG antibodies. Both, malaria-naive adults and malaria-exposed preschool children elicit vaccine-specific antibodies with broad inhibitory activity against geographically diverse P. falciparum isolates. Peptide-mapping studies of IgG subclass responses identified IgG3 against a peptide derived from MSP3 as the strongest predictor of antibody-dependent cellular inhibition.CONCLUSIONS:
These findings suggest that GMZ2 adjuvanted in Al(OH)3 elicits high levels of specific and functional antibodies with the capacity to control parasite multiplication.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Antibodies, Protozoan
/
Malaria, Falciparum
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Malaria Vaccines
Type of study:
Clinical_trials
/
Prognostic_studies
Limits:
Adult
/
Child, preschool
/
Humans
Language:
En
Journal:
J Infect Dis
Year:
2013
Type:
Article
Affiliation country:
Denmark