Marseillevirus-like virus recovered from blood donated by asymptomatic humans.
J Infect Dis
; 208(7): 1042-50, 2013 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-23821720
ABSTRACT
The study of the human virome is still in its infancy, especially with regard to the viral content of the blood of people who are apparently disease free. In this study, the genome of a new giant virus that is related to the amoeba-infecting pathogen Marseillevirus was recovered from donated blood, using high-throughput sequencing. Viral antigens were identified by an immunoconversion assay. The virus was visualized with transmission electron microscopy and fluorescence in situ hybridization and was grown in human T lymphocytes. Specific antibody reactions were used to identify viral proteins in blood specimens from polymerase chain reactive-positive donors. Finally, we tested 20 blood specimens from additional donors. Three had antibodies directed against this virus, and 2 had circulating viral DNA. This study shows that giant viruses, which are missed by the use of ultrafilters, are part of the human blood virome. The putative pathogenic role of giant viruses in humans remains undefined.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Blood
/
Blood Donors
/
Virus Diseases
/
DNA Viruses
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Infect Dis
Year:
2013
Type:
Article
Affiliation country:
France