An alternative beads-on-a-string chromatin architecture in Thermococcus kodakarensis.
EMBO Rep
; 14(8): 711-7, 2013 Aug.
Article
in En
| MEDLINE
| ID: mdl-23835508
ABSTRACT
We have applied chromatin sequencing technology to the euryarchaeon Thermococcus kodakarensis, which is known to possess histone-like proteins. We detect positioned chromatin particles of variable sizes associated with lengths of DNA differing as multiples of 30 bp (ranging from 30 bp to >450 bp) consistent with formation from dynamic polymers of the archaeal histone dimer. T. kodakarensis chromatin particles have distinctive underlying DNA sequence suggesting a genomic particle-positioning code and are excluded from gene-regulatory DNA suggesting a functional organization. Beads-on-a-string chromatin is therefore conserved between eukaryotes and archaea but can derive from deployment of histone-fold proteins in a variety of multimeric forms.
Full text:
1
Database:
MEDLINE
Main subject:
Histones
/
Nucleosomes
/
Thermococcus
/
Archaeal Proteins
/
DNA, Archaeal
/
Genome, Archaeal
Language:
En
Journal:
EMBO Rep
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Type:
Article
Affiliation country:
Japan