Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid-assisted flavoprotein strategy for regioselective aromatic hydroxylation.

Montersino, Stefania; Orru, Roberto; Barendregt, Arjan; Westphal, Adrie H; van Duijn, Esther; Mattevi, Andrea; van Berkel, Willem J H

Montersino, Stefania; Orru, Roberto; Barendregt, Arjan; Westphal, Adrie H; van Duijn, Esther; Mattevi, Andrea; van Berkel, Willem J H.

Affiliation

Montersino S; From the Laboratory of Biochemistry, Wageningen University, 6703 HA Wageningen, The Netherlands.
Orru R; Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy, and.
Barendregt A; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Westphal AH; From the Laboratory of Biochemistry, Wageningen University, 6703 HA Wageningen, The Netherlands.
van Duijn E; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Mattevi A; Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100 Pavia, Italy, and. Electronic address: andrea.mattevi@unipv.it.
van Berkel WJH; From the Laboratory of Biochemistry, Wageningen University, 6703 HA Wageningen, The Netherlands,. Electronic address: willem.vanberkel@wur.nl.

J Biol Chem ; 288(36): 26235-26245, 2013 Sep 06.

Article in En | MEDLINE | ID: mdl-23864660

Subject(s)

Bacterial Proteins/chemistry; Flavoproteins/chemistry; Mixed Function Oxygenases/chemistry; Phospholipids/chemistry; Rhodococcus/enzymology; Amino Acid Substitution; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; Crystallography, X-Ray; Escherichia coli/genetics; Escherichia coli/metabolism; Flavoproteins/genetics; Flavoproteins/metabolism; Gene Expression; Gentisates/chemistry; Gentisates/metabolism; Hydroxylation; Mass Spectrometry; Mixed Function Oxygenases/genetics; Mixed Function Oxygenases/metabolism; Mutation, Missense; NAD/chemistry; NAD/genetics; NAD/metabolism; Phospholipids/genetics; Phospholipids/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Rhodococcus/genetics

Key words

Crystal Structure; Flavoproteins; Gentisate; Hydroxylase; Monooxygenase; Oxidation-Reduction; Phospholipid; Rhodococcus

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Full text: 1 Database: MEDLINE Main subject: Phospholipids / Bacterial Proteins / Rhodococcus / Flavoproteins / Mixed Function Oxygenases Language: En Journal: J Biol Chem Year: 2013 Type: Article Affiliation country: Netherlands

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