Afrotheria genome; overestimation of genome size and distinct chromosome GC content revealed by flow karyotyping.
Genomics
; 102(5-6): 468-71, 2013.
Article
in En
| MEDLINE
| ID: mdl-24055950
ABSTRACT
Afrotheria genome size is reported to be over 50% larger than that of human, but we show that this is a gross overestimate. Although genome sequencing in Afrotheria is not complete, extensive homology with human has been revealed by chromosome painting. We provide new data on chromosome size and GC content in four Afrotherian species using flow karyotyping. Genome sizes are 4.13 Gb in aardvark, 4.01 Gb in African elephant, 3.69 Gb in golden mole and 3.31 Gb in manatee, whereas published results show a mean of 5.18 Gb for Afrotheria. Genome GC content shows a negative correlation with size, indicating that this is due to differences in the amount of AT-rich sequences. Low genome GC content and small variance in chromosome GC content are characteristic of aardvark and elephant and may be associated with the high degree of conserved synteny, suggesting that these are features of the Afrotherian ancestral genome.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Chromosomes, Mammalian
/
Genome Size
/
Mammals
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
2013
Type:
Article