Actins/metabolism; Epithelial Cells/drug effects; Epithelial Cells/enzymology; Gene Silencing/drug effects; Lens, Crystalline/cytology; Methionine Sulfoxide Reductases/metabolism; Peroxynitrous Acid/pharmacology; Enzyme Activation/drug effects; Extracellular Signal-Regulated MAP Kinases/metabolism; Fibroblast Growth Factor 2/pharmacology; Humans; Immunoblotting; Mitogen-Activated Protein Kinase Kinases/metabolism; Nitrosation/drug effects; Phosphorylation/drug effects; Signal Transduction/drug effects

DMEM; Dulbecco's Modified Eagle's Medium; ECL; ERK; Extracellular signal-regulated kinase; F-actin; G-actin; Human lens epithelial cells; MAPK; MEK; MetO; Methionine sulfoxide reductase B1; Msr; NCS; PBS; Peroxynitrite; RNS; ROS; RT; SelR; T-TBS; bFGF; basic fibroblast growth factor; enhanced chemiluminescence; extracellular signal-related kinase; filamentous actin; globular actin; hLE; human lens epithelial; methionine sulfoxide; methionine sulfoxide reductase; mitogen-activated protein kinase; mitogen-activated protein kinase kinase; newborn calf serum; phosphate-buffered saline; reactive nitrogen species; reactive oxygen species; room temperature; selenoprotein R; short interfering RNA; siRNA; tris-buffered saline with Tween