Benthic macroinvertebrate communities as aquatic bioindicators of contamination by Giardia and Cryptosporidium.

ABSTRACT

Benthic macroinvertebrates (community composed mostly by aquatic forms of insects, such as stonefly nymphs, dragonfly nymphs, water bugs or beetle larvae) are often used in biological monitoring programmes to evaluate the ecological status of rivers and thus to indicate the repercussions of anthropogenic activities. The aim of the present study was to evaluate the use of this indicator community to detect human enteroprotozoan parasites that are transmitted via water. In total, 32 samples of macroinvertebrates were collected, with the aid of surber nets of mesh size 500 µm, from nine rivers in Galicia (NW Spain), on different occasions between 2005 and 2009. The samples were homogenised (0.04 M phosphate buffered saline, pH 7.2), sieved (150 and 45 µm mesh), and concentrated (by a diphasic method). Aliquots of the sediments were then analysed by a direct immunofluorescence technique with monoclonal antibodies against Giardia and Cryptosporidium. Giardia cysts were detected in one (3.1%) of the samples and Cryptosporidium oocysts were detected in four (12.5%) of the samples. This work is the first study carried out to investigate the presence of Giardia and Cryptosporidium in this benthic community. The results demonstrate that benthic invertebrates could be used as bioindicators of contamination by these waterborne protozoans. Moreover, as this aquatic organisms act as intermittent accumulators and its monitoring enables chronological analysis of perturbations, in both the short- and mid-term, this may represent a suitable alternative or complementary method to the usual techniques of detecting human and animal enteropathogens in water samples.