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Resolution as a function of accelerating voltage in electron microscopy of semithick biological specimens.
Cole, R W; Parsons, D F.
Affiliation
  • Cole RW; High-Voltage Electron Microscope Biotechnology Resource, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Cell Biophys ; 13(2): 133-40, 1988 Oct.
Article in En | MEDLINE | ID: mdl-2464433
In the past, biological sections ranging in thickness from 0.10- to 0.50-micron have usually been examined with high-voltage (greater than 500 kV) electron microscopes (HVEM). Now investigators are increasingly using intermediate voltage (200-500 kV) electron microscopes (IVEM), which are more readily available and demand less maintenance. In a study of "typical" plastic-embedded, stained sections of mouse liver ranging from 0.10 to 1.0 micron thick, we determined the resolution obtainable at 100, 200, and 1000 kV. At all three accelerating voltages the resolution (2.7 nm) for 0.10-micron sections was limited only by the sections stain granularity. For 0.25-micron thickness the resolutions were 5.8, 3.1, and 3.1 nm at 100, 200, and 1000 kV, respectively. The maximum usable thickness at 200 kV with resolution sufficient to resolve membranes clearly was between 0.75 and 1.0 micron, depending on the magnification. Resolution at 100 kV was adequate for screening sections up to 1.0-micron thick for preparation defects prior to examination with an IVEM or HVEM.
Subject(s)
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Database: MEDLINE Main subject: Microscopy, Electron / Liver Limits: Animals Language: En Journal: Cell Biophys Year: 1988 Type: Article
Search on Google
Database: MEDLINE Main subject: Microscopy, Electron / Liver Limits: Animals Language: En Journal: Cell Biophys Year: 1988 Type: Article