Mechanistic studies on the flavin-dependent N6-lysine monooxygenase MbsG reveal an unusual control for catalysis.

Robinson, Reeder M; Rodriguez, Pedro J; Sobrado, Pablo

Robinson, Reeder M; Rodriguez, Pedro J; Sobrado, Pablo.

Affiliation

Robinson RM; Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA; Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.
Rodriguez PJ; Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA; Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.
Sobrado P; Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA; Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA; Virginia Tech Center for Drug Discovery, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: psobrado@vt.edu.

Arch Biochem Biophys ; 550-551: 58-66, 2014 May 15.

Article in En | MEDLINE | ID: mdl-24769337

Subject(s)

Bacterial Proteins/metabolism; Dinitrocresols/metabolism; Lysine/metabolism; Mixed Function Oxygenases/metabolism; Mycobacterium smegmatis/chemistry; NADP/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Biocatalysis; Flavins; Hydrogen Peroxide/metabolism; Hydrogen-Ion Concentration; Kinetics; Mixed Function Oxygenases/chemistry; Mixed Function Oxygenases/genetics; Mycobacterium smegmatis/enzymology; Oxidation-Reduction; Protein Binding; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Superoxides/metabolism

Key words

Conformational changes; Flavin-dependent monooxygenases; Rapid reaction kinetics; Siderophores; Viscosity effects; pH studies

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Database: MEDLINE Main subject: Bacterial Proteins / Mycobacterium smegmatis / Dinitrocresols / Mixed Function Oxygenases / Lysine / NADP Language: En Journal: Arch Biochem Biophys Year: 2014 Type: Article Affiliation country: United States

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google